DIRECT-DETECTION OF LISTERIA-MONOCYTOGENES IN 25 MILLILITERS OF RAW-MILK BY A 2-STEP PCR WITH NESTED PRIMERS

被引：71
作者：
HERMAN, LMF
DEBLOCK, JHGE
MOERMANS, RJB
机构：
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1995年 / 61卷 / 02期
关键词：
D O I：
10.1128/AEM.61.2.817-819.1995
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A method for direct detection of Listeria monocytogenes in 25 ml of raw milk is presented. The detection limit can be situated between 10 and 5 CFU. The detection method is based on chemical extraction of the milk components and PCR amplification with two nested pairs of primers specific for Listeria monocytogenes.
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页码：817 / 819
页数：3
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