The investigation was undertaken for standardising an efficient and reproducible tissue culture system for subsequent improvement programmes through somaclonal variation in forage sorghum, Sorghum bicolor (L.) Moench. Immature inflorescences of a pureline derivative from Sorghum x Sudan grass hybridization SSG-593 were used. Segments of inflorescence plated on modified LS produced profuse callus (81%) which on transfer to modified MS showed 47-95% plantlet regeneration. Five weeks old callus gave 100% plant regeneration which decreased with ageing of the callus. Anatomical examination of the regenerating callus suggested that plant regeneration occured through embryogenesis.
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UNIV PARIS 11, INST BIOTECHNOL PLANTES, URA CNRS D1128, F-91405 ORSAY, FRANCEUNIV PARIS 11, INST BIOTECHNOL PLANTES, URA CNRS D1128, F-91405 ORSAY, FRANCE
Gendy, C
Sene, M
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UNIV PARIS 11, INST BIOTECHNOL PLANTES, URA CNRS D1128, F-91405 ORSAY, FRANCEUNIV PARIS 11, INST BIOTECHNOL PLANTES, URA CNRS D1128, F-91405 ORSAY, FRANCE
Sene, M
VanLe, B
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UNIV PARIS 11, INST BIOTECHNOL PLANTES, URA CNRS D1128, F-91405 ORSAY, FRANCEUNIV PARIS 11, INST BIOTECHNOL PLANTES, URA CNRS D1128, F-91405 ORSAY, FRANCE
VanLe, B
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Vidal, J
Van, KTT
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UNIV PARIS 11, INST BIOTECHNOL PLANTES, URA CNRS D1128, F-91405 ORSAY, FRANCEUNIV PARIS 11, INST BIOTECHNOL PLANTES, URA CNRS D1128, F-91405 ORSAY, FRANCE