EMBRYOGENIC CALLUS INDUCTION AND PLANT-REGENERATION IN FORAGE SORGHUM SORGHUM-BICOLOR (L) MOENCH

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作者
ARTI, P
DEVI, DR
MURTY, UR
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S3 [农学(农艺学)];
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0901 ;
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The investigation was undertaken for standardising an efficient and reproducible tissue culture system for subsequent improvement programmes through somaclonal variation in forage sorghum, Sorghum bicolor (L.) Moench. Immature inflorescences of a pureline derivative from Sorghum x Sudan grass hybridization SSG-593 were used. Segments of inflorescence plated on modified LS produced profuse callus (81%) which on transfer to modified MS showed 47-95% plantlet regeneration. Five weeks old callus gave 100% plant regeneration which decreased with ageing of the callus. Anatomical examination of the regenerating callus suggested that plant regeneration occured through embryogenesis.
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页码:71 / 77
页数:7
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