CHEMICAL ATTACHMENT OF ORGANOMETALLICS TO PROTEINS IN REVERSE MICELLES

被引：0
|
作者：
RYABOV, AD
TRUSHKIN, AM
BAKSHEEVA, LI
GORBATOVA, RK
KUBRAKOVA, IV
MOZHAEV, VV
GNEDENKO, BB
LEVASHOV, AV
机构：
[1] RUSSIAN ACAD SCI,INST GEOCHEM & ANALYT CHEM,MOSCOW,USSR
[2] UNIV WITTEN HERDECKE,INST ANORGAN CHEM,W-5810 WITTEN,GERMANY
[3] MV LOMONOSOV STATE UNIV,DEPT CHEM,MOSCOW 119899,USSR
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH | 1992年 / 31卷 / 06期
关键词：
D O I：
10.1002/anie.199207891
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
For the construction of new biocatalysts is might be necessary to attach a water-insoluble organometallic residue covalently to a soluble enzyme. Reverse micelles "microreactors" with a polar center and a nonpolar shell-can achieve this, as shown by the covalent binding of carboxyferrocene (Fc-COOH) to glucose oxidase (E-NH2) in the presence of dicyclohexylcarbodiimide N-hyddroxysuccinimide as activating couple [Eq. (a)] in a system containing borate buffer, n-octane, aerosol OT, and acetonitrile.
引用
收藏
页码：789 / 791
页数：3
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