CHEMICAL ATTACHMENT OF ORGANOMETALLICS TO PROTEINS IN REVERSE MICELLES

被引：0

作者：

RYABOV, AD

TRUSHKIN, AM

BAKSHEEVA, LI

GORBATOVA, RK

KUBRAKOVA, IV

MOZHAEV, VV

GNEDENKO, BB

LEVASHOV, AV

机构：

[1] RUSSIAN ACAD SCI,INST GEOCHEM & ANALYT CHEM,MOSCOW,USSR

[2] UNIV WITTEN HERDECKE,INST ANORGAN CHEM,W-5810 WITTEN,GERMANY

[3] MV LOMONOSOV STATE UNIV,DEPT CHEM,MOSCOW 119899,USSR

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH | 1992年 / 31卷 / 06期

关键词：

D O I：

10.1002/anie.199207891

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

For the construction of new biocatalysts is might be necessary to attach a water-insoluble organometallic residue covalently to a soluble enzyme. Reverse micelles "microreactors" with a polar center and a nonpolar shell-can achieve this, as shown by the covalent binding of carboxyferrocene (Fc-COOH) to glucose oxidase (E-NH2) in the presence of dicyclohexylcarbodiimide N-hyddroxysuccinimide as activating couple [Eq. (a)] in a system containing borate buffer, n-octane, aerosol OT, and acetonitrile.

引用

页码：789 / 791

页数：3

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