CENTROMERIC DNA OF KLUYVEROMYCES-LACTIS

被引：61
作者：
HEUS, JJ
ZONNEVELD, BJM
STEENSMA, HY
VANDENBERG, JA
机构：
[1] Department of Cell Biology and Genetics, Leiden University, Leiden, NL-2333 AL
来源：
CURRENT GENETICS | 1990年 / 18卷 / 06期
关键词：
ISOLATION; CENTROMERES; KLUYVEROMYCES-LACTIS;
D O I：
10.1007/BF00327022
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
A direct selection method was used to isolate centromeres from a genomic library of the yeast Kluyveromyces lactis. The method is based on the lethality at high copy number of the ochre-suppressing tRNA gene SUP11. Five different chromosomal fragments were found that confer mitotic stability to plasmids containing a replication origin of K. lactis (KARS). In addition, KARS plasmids containing these fragments have a copy number of approximately one, and each of the five fragments hybridizes to a different chromosome of K. lactis. From these results we conclude that five of the six centromeres of K. lactis have been isolated. These centromeres do not function in S. cerevisiae.
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页码：517 / 522
页数：6
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