NONISOTOPIC INSITU HYBRIDIZATION AS A METHOD FOR NONDISJUNCTION STUDIES IN HUMAN SPERMATOZOA

被引：55

作者：

COONEN, E ^{[1
]}

PIETERS, MHEC ^{[1
]}

DUMOULIN, JCM ^{[1
]}

MEYER, H ^{[1
]}

EVERS, JLH ^{[1
]}

RAMAEKERS, FCS ^{[1
]}

GERAEDTS, JPM ^{[1
]}

机构：

[1] STATE UNIV LIMBURG,DEPT OBSTET GYNAECOL,6200 MD MAASTRICHT,NETHERLANDS

来源：

MOLECULAR REPRODUCTION AND DEVELOPMENT | 1991年 / 28卷 / 01期

关键词：

D O I：

10.1002/mrd.1080280104

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human spermatozoa were studied with a nonradioactive in situ hybridization method. Using a chemically modified DNA probe and immunocytochemical reactions for visualization, it was possible to obtain hybridization signals in 31 of 32 semen samples. Positive hybridization reactions, depending on cell accessibility, varied from 40% to over 90% for the different samples. Using a chromosome 1‐specific DNA probe, disomy for this chromosome was found in 0.67% of all accessible sperm cells. Copyright © 1991 Wiley‐Liss, Inc.

引用

页码：18 / 22

页数：5

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