PEPTIDYL-TRNA .7. SUBSTRATE SPECIFICITY OF PEPTIDYL-TRNA HYDROLASE

被引：28
作者：
DEGROOT, N
GRONER, Y
LAPIDOT, Y
机构：
[1] Department of Biological Chemistry, The Hebrew University of Jerusalen, Jerusalem
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 186卷 / 02期
关键词：
D O I：
10.1016/0005-2787(69)90006-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The enzymatic hydrolysis of peptidyl-tRNA is described. The hydrolysis rates of peptidyl-tRNA with different peptide chain lengths containing free and blocked α-amino groups are compared to the hydrolysis rates of N-acylaminoacyl-tRNA. It is shown that the hydrolysis rate of peptidyl-tRNA containing two peptide bonds is considerably higher than that of N-acylaminoacyl-tRNA. Moreover, the hydrolysis rate of different peptidyl-tRNA's depends on the peptide chain length. Thus, Gly2-Phe-tRNA is hydrolyzed faster than GlyPhe-tRNA, and Gly4Phe-tRNA is hydrolyzed faster than Gly2Phe-tRNA. The apparent Km and vmax values for Ac-Leu-tRNA are compared to those of Ala2Leu-tRNA. © 1969.
引用
收藏
页码：286 / &
相关论文
共 18 条
[1]   USE OF ESTERS OF N-HYDROXYSUCCINIMIDE IN PEPTIDE SYNTHESIS [J].
ANDERSON, GW ;
CALLAHAN, FM ;
ZIMMERMAN, JE .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1964, 86 (09) :1839-+
[2]  
BERGMANN F, 1961, J BIOL CHEM, V236, P1735
[3]   A SIMPLE EFFICIENT LIQUID SCINTILLATOR FOR COUNTING AQUEOUS SOLUTIONS IN A LIQUID SCINTILLATION COUNTER [J].
BRAY, GA .
ANALYTICAL BIOCHEMISTRY, 1960, 1 (4-5) :279-285
[4]   ENZYMATIC HYDROLYSIS OF N-SUBSTITUTED AMINOACYL-TRNA [J].
CUZIN, F ;
KRETCHMER, N ;
GREENBERG, RE ;
HURWITZ, R ;
CHAPEVILLE, F .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1967, 58 (05) :2079-+
[5]  
DEGROOT N, 1968, BIOCHEM BIOPHYS RES, V31, P39
[6]  
GRONER J, 1968, ISRAEL J CHEM, V6, pP96
[7]   STUDIES ON POLYNUCLEOTIDES .86. ENZYMIC HYDROLYSIS OF N-ACYLAMINOACYL-TRANSFER RNA [J].
KOSSEL, H ;
RAJBHANDARY, UL .
JOURNAL OF MOLECULAR BIOLOGY, 1968, 35 (03) :539-+
[8]   A CHEMICAL SYNTHESIS OF GLYCYLGLYCYL[14C]PHENYLALANYL-TRNA AND GLYCYLGLYCYLGLYCYL[14C]PHENYLALANYL-TRNA [J].
LAPIDOT, Y ;
DEGROOT, N ;
RAPPOPORT, S ;
PANET, A .
BIOCHIMICA ET BIOPHYSICA ACTA, 1968, 157 (02) :433-+
[9]   MODIFIED AMINOACYL-TRNA .3. A GENERAL PROCEDURE FOR SYNTHESIS OF DIPEPTIDYL TRANSFER RNA [J].
LAPIDOT, Y ;
DEGROOT, N ;
RAPPOPORT, S ;
HAMBURGER, AD .
BIOCHIMICA ET BIOPHYSICA ACTA, 1967, 149 (02) :532-+
[10]   MODIFIED AMINOACYL-TRNA .2. A GENERAL METHOD FOR PREPARATION OF ACYLAMINOACYL-TRNA [J].
LAPIDOT, Y ;
DEGROOT, N ;
FRYSHAFR.I .
BIOCHIMICA ET BIOPHYSICA ACTA, 1967, 145 (02) :292-&
12