MODIFICATION OF PLASMA-PROTEINS BY CIGARETTE-SMOKE AS MEASURED BY PROTEIN CARBONYL FORMATION

被引:233
作者
REZNICK, AZ
CROSS, CE
HU, ML
SUZUKI, YJ
KHWAJA, S
SAFADI, A
MOTCHNIK, PA
PACKER, L
HALLIWELL, B
机构
[1] UNIV CALIF DAVIS, MED CTR,DIV PULM CRIT CARE MED,4301 X ST, ROOM 2120, SACRAMENTO, CA 95817 USA
[2] UNIV CALIF BERKELEY, DEPT BIOCHEM & MOLEC BIOL, BERKELEY, CA 94720 USA
[3] UNIV CALIF BERKELEY, MEMBRANE BIOENERGET GRP, BERKELEY, CA 94720 USA
关键词
D O I
10.1042/bj2860607
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Exposure of human plasma to gas-phase (but not to whole) cigarette smoke (CS) produces oxidative damage to lipids [Frei, Forte, Ames & Cross (1991) Biochem. J. 277, 133-138], which is prevented by ascorbic acid. The ability of CS to induce protein damage was measured by the carbonyl assay and by loss of enzyme activity and protein-SH groups. Both whole and gas-phase CS caused formation of carbonyls in human plasma, which was partially inhibited by GSH but not by ascorbic acid or metal-ion-chelating agents. Isolated albumin exposed to CS showed much faster carbonyl formation (per unit protein) than did whole plasma; damage to isolated albumin was partially prevented by chelating agents. Isolated creatine kinase (CK) lost activity upon exposure to CS much faster than did CK in plasma. Direct addition to plasma of mixtures of some or all of the aldehydes reported to be present in CS caused protein carbonyl formation and inactivation of CK, but neither occurred to the extent produced by CS exposure.
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页码:607 / 611
页数:5
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