AN ATTEMPT TO IMPROVE THE SOS CHROMOTEST RESPONSES

被引：5
作者：
HOFLACK, JC
FERARD, JF
VASSEUR, P
BLAISE, C
机构：
[1] UNIV METZ,CTR SCI ENVIRONNEMENT,1 RUE RECOLLETS,F-57000 METZ,FRANCE
[2] ENVIRONNEMENT CANADA,CTR ST LAURENT,LONGUEUIL J4K 1A1,PQ,CANADA
来源：
JOURNAL OF APPLIED TOXICOLOGY | 1993年 / 13卷 / 05期
关键词：
ARTIFACTS; COMPLEX MIXTURES; CYTOTOXICITY; GENOTOXICITY; INDUSTRIAL WASTE; SOS CHROMOTEST;
D O I：
10.1002/jat.2550130504
中图分类号：
R99 [毒物学（毒理学）];
学科分类号：
100405 ;
摘要：
The SOS Chromotest was carried out on leachates of ten industrial wastes with the standard procedure and a miniaturized version with microplates. The two methods gave identical results in nine samples (eight negative and one positive). A simple additional manipulation is described for the identification of the false positive response that is frequently observed with complex mixtures. It involves challenging the SOS Chromotest bacteria with samples (having previously shown a positive genotoxic response) just before the enzymatic activities (i.e. beta-galactosidase and alkaline phosphatase) are estimated colorimetrically. This additional step eliminates discrepancies between the results for the standard and the miniaturized procedures.
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页码：315 / 319
页数：5
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