PURIFICATION AND CHARACTERIZATION OF COPPER-METALLOTHIONEINS FROM ASPERGILLUS-NIGER

被引：12

作者：

KERMASHA, S ^{[1
]}

PELLERIN, F ^{[1
]}

ROVEL, B ^{[1
]}

GOETGHEBEUR, M ^{[1
]}

METCHE, M ^{[1
]}

机构：

[1] INST NATL POLYTECH LORRAINE, BIOCHIM APPL LAB, F-54505 VANDOEUVRE LES NANCY, FRANCE

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1993年 / 57卷 / 09期

关键词：

D O I：

10.1271/bbb.57.1420

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Copper metallothioneins were extracted from Aspergillus niger after an induction period of five hours in a copper-containing suspension medium. The crude extract (FI) was obtained by cell homogenization and the partially purified extract (FII) was obtained by heat treatment at 60-degrees-C for 10 min. The partially purified extract (FII) was further purified by successive chromatographies on size-exclusion (Biogel P-30) and ion-exchange (TSK-HW 65-DEAE) chromatography columns. All protein fractions were analyzed for their protein and induced copper contents. The Cu/protein ratio increased steadily throughout the purification process; the most purified fraction (FIV) showed a 24-fold increase, with a recovery of 13%. The molecular weight of the Cu-metallothionein fraction FIV was 11,000 and the ratio of moles of copper per mole of protein was 6.6.

引用

页码：1420 / 1423

页数：4

共 25 条

[1] SPECTROSCOPIC STUDIES ON NEUROSPORA COPPER METALLOTHIONEIN [J].

BELTRAMINI, M ;

LERCH, K .

BIOCHEMISTRY, 1983, 22 (09) :2043-2048

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3]

BREMNER I, 1991, METHOD ENZYMOL, V205, P25

[4] DISTRIBUTION OF COPPER AND ZINC IN LIVER OF DEVELOPING SHEEP FETUS [J].

BREMNER, I ;

WILLIAMS, RB ;

YOUNG, BW .

BRITISH JOURNAL OF NUTRITION, 1977, 38 (01) :87-92

[5] YEAST METALLOTHIONEIN AND APPLICATIONS IN BIOTECHNOLOGY [J].

BUTT, TR ;

ECKER, DJ .

MICROBIOLOGICAL REVIEWS, 1987, 51 (03) :351-364

[6] RESISTANCE OF CULTURED HEPATOMA-CELLS TO COPPER TOXICITY - PURIFICATION AND CHARACTERIZATION OF THE HEPATOMA METALLOTHIONEIN [J].

FREEDMAN, JH ;

PEISACH, J .

BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 992 (02) :145-154

[7] METALLOTHIONEIN [J].

HAMER, DH .

ANNUAL REVIEW OF BIOCHEMISTRY, 1986, 55 :913-951

[8] COPPER-THIONEIN FROM FETAL BOVINE LIVER [J].

HARTMANN, HJ ;

WESER, U .

BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 491 (01) :211-222

[9] COPPER-INDUCED BINDING OF CELLULAR FACTORS TO YEAST METALLOTHIONEIN UPSTREAM ACTIVATION SEQUENCES [J].

HUIBREGTSE, JM ;

ENGELKE, DR ;

THIELE, DJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (01) :65-69

[10]

KAGI JHR, 1974, J BIOL CHEM, V249, P3537

← 1 2 3 →