UV-C SENSITIVITY OF UNSTIMULATED AND STIMULATED HUMAN-LYMPHOCYTES FROM NORMAL AND XERODERMA-PIGMENTOSUM DONORS IN THE COMET ASSAY - A POTENTIAL DIAGNOSTIC-TECHNIQUE

被引：140

作者：

GREEN, MHL ^{[1
]}

LOWE, JE ^{[1
]}

HARCOURT, SA ^{[1
]}

AKINLUYI, P ^{[1
]}

ROWE, T ^{[1
]}

COLE, J ^{[1
]}

ANSTEY, AV ^{[1
]}

ARLETT, CF ^{[1
]}

机构：

[1] ST BARTHOLOMEWS HOSP,DEPT DERMATOL,LONDON EC1A 7BE,ENGLAND

来源：

MUTATION RESEARCH | 1992年 / 273卷 / 02期

关键词：

COMET ASSAY; DIAGNOSIS; HUMAN LYMPHOCYTES; UV-C; XERODERMA PIGMENTOSUM;

D O I：

10.1016/0921-8777(92)90075-E

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have studied incision-break formation in unstimulated and stimulated populations of human T-lymphocytes using the comet (single-cell microgel electrophoresis) assay. The frequency of strand breaks 1 h after UV-irradiation appears to be far greater in unstimulated than in stimulated lymphocytes from normal donors and the excess of strand breaks was observed for a far longer time after irradiation. This result corroborates the greater sensitivity to UV-C irradiation observed in a colony-forming assay but suggests that the defect may relate to a defect in strand rejoining rather than a defect in incision. Few strand breaks were seen in either unstimulated or stimulated lymphocytes of four xeroderma pigmentosum donors, suggesting that the method may offer a rapid diagnostic assay for XP.

引用

页码：137 / 144

页数：8

共 15 条

[1]

ARLETT CF, 1980, CANCER RES, V40, P926

[2] DEFECTIVE REPAIR REPLICATION OF DNA IN XERODERMA PIGMENTOSUM [J].

CLEAVER, JE .

NATURE, 1968, 218 (5142) :652-&

[3] COMPARATIVE HUMAN CELLULAR RADIOSENSITIVITY .2. THE SURVIVAL FOLLOWING GAMMA-IRRADIATION OF UNSTIMULATED (G0) LYMPHOCYTES-T, LYMPHOCYTE-T LINES, LYMPHOBLASTOID CELL-LINES AND FIBROBLASTS FROM NORMAL DONORS, FROM ATAXIA-TELANGIECTASIA PATIENTS AND FROM ATAXIA-TELANGIECTASIA HETEROZYGOTESC [J].

COLE, J ;

ARLETT, CF ;

GREEN, MHL ;

HARCOURT, SA ;

PRIESTLEY, A ;

HENDERSON, L ;

COLE, H ;

JAMES, SE ;

RICHMOND, F .

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1988, 54 (06) :929-943

[4]

COOK PR, 1975, J CELL SCI, V19, P261

[5] EXCISION REPAIR OF PYRIMIDINE DIMERS IN HUMAN PERIPHERAL-BLOOD LYMPHOCYTES - COMPARISON BETWEEN MITOGEN STIMULATED AND UNSTIMULATED CELLS [J].

FREEMAN, SE ;

RYAN, SL .

MUTATION RESEARCH, 1988, 194 (02) :143-150

[6]

HENDERSON EE, 1983, P SOC EXP BIOL MED, V172, P524

[7] DNA-REPAIR PROTECTS AGAINST CUTANEOUS AND INTERNAL NEOPLASIA - EVIDENCE FROM XERODERMA PIGMENTOSUM [J].

KRAEMER, KH ;

LEE, MM ;

SCOTTO, J .

CARCINOGENESIS, 1984, 5 (04) :511-514

[8] XERODERMA-PIGMENTOSUM - CUTANEOUS, OCULAR, AND NEUROLOGIC ABNORMALITIES IN 830 PUBLISHED CASES [J].

KRAEMER, KH ;

LEE, MM ;

SCOTTO, J .

ARCHIVES OF DERMATOLOGY, 1987, 123 (02) :241-250

[9]

KUTLACA R, 1982, MUTAT RES, V94, P125

[10] DNA-REPAIR AND CANCER - SPECULATIONS BASED ON STUDIES WITH XERODERMA PIGMENTOSUM, COCKAYNES SYNDROME AND TRICHOTHIODYSTROPHY [J].

LEHMANN, AR ;

NORRIS, PG .

CARCINOGENESIS, 1989, 10 (08) :1353-1356

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