UV-C SENSITIVITY OF UNSTIMULATED AND STIMULATED HUMAN-LYMPHOCYTES FROM NORMAL AND XERODERMA-PIGMENTOSUM DONORS IN THE COMET ASSAY - A POTENTIAL DIAGNOSTIC-TECHNIQUE

被引：140

作者：

GREEN, MHL ^{[1
]}

LOWE, JE ^{[1
]}

HARCOURT, SA ^{[1
]}

AKINLUYI, P ^{[1
]}

ROWE, T ^{[1
]}

COLE, J ^{[1
]}

ANSTEY, AV ^{[1
]}

ARLETT, CF ^{[1
]}

机构：

[1] ST BARTHOLOMEWS HOSP,DEPT DERMATOL,LONDON EC1A 7BE,ENGLAND

来源：

MUTATION RESEARCH | 1992年 / 273卷 / 02期

关键词：

COMET ASSAY; DIAGNOSIS; HUMAN LYMPHOCYTES; UV-C; XERODERMA PIGMENTOSUM;

D O I：

10.1016/0921-8777(92)90075-E

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have studied incision-break formation in unstimulated and stimulated populations of human T-lymphocytes using the comet (single-cell microgel electrophoresis) assay. The frequency of strand breaks 1 h after UV-irradiation appears to be far greater in unstimulated than in stimulated lymphocytes from normal donors and the excess of strand breaks was observed for a far longer time after irradiation. This result corroborates the greater sensitivity to UV-C irradiation observed in a colony-forming assay but suggests that the defect may relate to a defect in strand rejoining rather than a defect in incision. Few strand breaks were seen in either unstimulated or stimulated lymphocytes of four xeroderma pigmentosum donors, suggesting that the method may offer a rapid diagnostic assay for XP.

引用

页码：137 / 144

页数：8