AN EXPERIMENTAL APPROACH TO PH MEASUREMENT IN THE INTERCELLULAR FREE SPACE OF HIGHER-PLANT TISSUES

被引：6

作者：

BALLARINDENTI, A ^{[1
]}

ANTONIOTTI, D ^{[1
]}

机构：

[1] CONSORZIO MILANO RIC,MILAN,ITALY

来源：

EXPERIENTIA | 1991年 / 47卷 / 05期

关键词：

PH; MICROELECTRODES; FREE SPACE; PLANTS; PARENCHYMA; APOPLAST;

D O I：

10.1007/BF01959949

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

An experimental approach to the measurement of pH in the free space of plant tissues was made, using both metal minielectrodes and H+-sensitive resin glass microelectrodes inserted in small environments artifically created inside the tissue. Experiments were carried out on the parenchyma of higher plants (corn, potato, squash, Senecio herraianus), monitoring pH changes in the free space as a function of the pH of the external medium. The pH in the free space has been found to be different from that of the surrounding solution and its variation in time much slower than would be predicted by assuming that simple diffusion of H+ ions took place. The results agree with the hypothesis that the free space behaves as a compartment distinct from both the cytosol and the surrounding medium.

引用

页码：478 / 482

页数：5

共 16 条

[1] EVIDENCE THAT ACID-SOLUTIONS INDUCE PLANT-CELL ELONGATION BY ACIDIFYING THE CYTOSOL AND STIMULATING THE PROTON PUMP [J].