RIBOTYPING OF BIFIDOBACTERIUM STRAINS USING CELLS EMBEDDED IN AGAROSE PLUGS AND A 16S RDNA PROBE

被引：12

作者：

MCCARTNEY, AL ^{[1
]}

TANNOCK, GW ^{[1
]}

机构：

[1] UNIV OTAGO,DEPT MICROBIOL,DUNEDIN,NEW ZEALAND

来源：

MICROBIAL ECOLOGY IN HEALTH AND DISEASE | 1995年 / 8卷 / 02期

关键词：

BIFIDOBACTERIA; RIBOTYPING;

D O I：

10.3109/08910609509141386

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Thirty-five strains of Bifidobacterium species obtained from Type Culture collections, or isolated from the faeces of human subjects, were differentiated using an improved ribotyping method in which bifidobacterial cells were embedded in agarose plugs prior to lysis. Twenty ribotypes were detected among the 35 isolates on the basis of BamHI, NarI, and PvuII digests of bifidobacterial DNA hybridised to a P-32-labelled 16S rDNA probe amplified from B. infantis ATCC 15697.

引用

页码：79 / 84

页数：6

共 20 条

[1] AN ELECTIVE AND SELECTIVE ISOLATION MEDIUM FOR BIFIDOBACTERIUM SPP [J].