RAPID PURIFICATION METHOD FOR HUMAN RECOMBINANT TUMOR-NECROSIS-FACTOR-ALPHA

被引：6
|
作者：
PAQUET, A [1 ]
LEVESQUE, A [1 ]
PAGE, M [1 ]
机构：
[1] UNIV LAVAL,FAC MED,DEPT BIOCHEM,ST FOY G1K 7P4,PQ,CANADA
来源：
JOURNAL OF CHROMATOGRAPHY A | 1994年 / 667卷 / 1-2期
关键词：
D O I：
10.1016/0021-9673(94)89060-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Human recombinant tumor necrosis factor alpha was purified in a single step to about 95% purity from Escherichia coli lysate by chromatography on hydroxyapatite. The last traces of contaminants were removed by fast protein liquid chromatography on a Mono Q column. The final product was found to be pure by gel electrophoresis with silver staining. A molecular mass of approximately 17 000 and a specific activity of 4.3 . 10(6) U/mg after a single purification step were found.
引用
收藏
页码：125 / 130
页数：6
相关论文
共 50 条
  • [41] PHASE-I TRIAL OF RECOMBINANT HUMAN TUMOR-NECROSIS-FACTOR-ALPHA IN PATIENTS WITH ADVANCED MALIGNANCY
    GAMM, H
    LINDEMANN, A
    MERTELSMANN, R
    HERRMANN, F
    EUROPEAN JOURNAL OF CANCER, 1991, 27 (07) : 856 - 863
  • [42] EXPERIMENTAL TREATMENT OF EQUINE SARCOID USING A XANTHATE COMPOUND AND RECOMBINANT HUMAN TUMOR-NECROSIS-FACTOR-ALPHA
    OTTEN, N
    MARTI, E
    SODERSTROM, C
    AMTMANN, E
    BURGER, D
    GERBER, H
    LAZARY, S
    JOURNAL OF VETERINARY MEDICINE SERIES A-PHYSIOLOGY PATHOLOGY CLINICAL MEDICINE, 1994, 41 (10): : 757 - 765
  • [43] THE INFLUENCE OF RECOMBINANT HUMAN INTERLEUKIN-1, TUMOR-NECROSIS-FACTOR-ALPHA AND TUMOR NECROSIS FACTOR-BETA ON HUMAN LYMPHOCYTE-T COLONY FORMATION
    ZUCALI, JR
    ELFENBEIN, GJ
    BARTH, KC
    DINARELLO, CA
    JOURNAL OF LEUKOCYTE BIOLOGY, 1987, 42 (04) : 331 - 331
  • [44] CYTOTOXIC MECHANISM OF TUMOR-NECROSIS-FACTOR-ALPHA
    LARRICK, JW
    WRIGHT, SC
    FASEB JOURNAL, 1990, 4 (14): : 3215 - 3223
  • [45] RECOMBINANT HUMAN TUMOR-NECROSIS-FACTOR-ALPHA (RTNF) STIMULATES THE SECRETION OF GRANULOCYTE COLONY-STIMULATING FACTOR INVIVO
    FURMAN, WL
    PRATT, CB
    LUMM, W
    IHLE, J
    PEDIATRIC RESEARCH, 1991, 29 (04) : A140 - A140
  • [46] EFFECT OF TUMOR-NECROSIS-FACTOR-ALPHA AND TUMOR-NECROSIS-FACTOR-BETA ON HUMAN OLIGODENDROCYTES AND NEURONS IN CULTURE
    MCLAURIN, J
    DSOUZA, S
    STEWART, J
    BLAIN, M
    BEAUDET, A
    NALBANTOGLU, J
    ANTEL, JP
    INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE, 1995, 13 (3-4) : 369 - 381
  • [47] TUMOR-NECROSIS-FACTOR-ALPHA IN NASAL ALLERGY
    HISAMATSU, K
    GANBO, T
    NAKAZAWA, T
    NAKAJIMA, T
    KO, J
    GOTO, R
    MURAKAMI, Y
    MITSUI, K
    MEDIATORS OF INFLAMMATION, 1995, 4 (05) : 368 - 373
  • [48] PRODUCTION OF TUMOR-NECROSIS-FACTOR-ALPHA AND HEMODIALYSIS
    MACDONALD, C
    RUSH, DN
    BERNSTEIN, KN
    MCKENNA, RM
    NEPHRON, 1993, 65 (02) : 273 - 277
  • [49] TUMOR-NECROSIS-FACTOR-ALPHA IN MALIGNANT DISEASE
    ABRAHAMSSON, J
    CARLSSON, B
    MELLANDER, L
    AMERICAN JOURNAL OF PEDIATRIC HEMATOLOGY ONCOLOGY, 1993, 15 (04): : 364 - 369
  • [50] THE MOLECULAR ACTION OF TUMOR-NECROSIS-FACTOR-ALPHA
    CAMUSSI, G
    ALBANO, E
    TETTA, C
    BUSSOLINO, F
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 202 (01): : 3 - 14
12345