RAPID PURIFICATION METHOD FOR HUMAN RECOMBINANT TUMOR-NECROSIS-FACTOR-ALPHA

被引：6
|
作者：
PAQUET, A [1 ]
LEVESQUE, A [1 ]
PAGE, M [1 ]
机构：
[1] UNIV LAVAL,FAC MED,DEPT BIOCHEM,ST FOY G1K 7P4,PQ,CANADA
来源：
JOURNAL OF CHROMATOGRAPHY A | 1994年 / 667卷 / 1-2期
关键词：
D O I：
10.1016/0021-9673(94)89060-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Human recombinant tumor necrosis factor alpha was purified in a single step to about 95% purity from Escherichia coli lysate by chromatography on hydroxyapatite. The last traces of contaminants were removed by fast protein liquid chromatography on a Mono Q column. The final product was found to be pure by gel electrophoresis with silver staining. A molecular mass of approximately 17 000 and a specific activity of 4.3 . 10(6) U/mg after a single purification step were found.
引用
收藏
页码：125 / 130
页数：6
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