RAPID PURIFICATION METHOD FOR HUMAN RECOMBINANT TUMOR-NECROSIS-FACTOR-ALPHA

被引：6
|
作者：
PAQUET, A [1 ]
LEVESQUE, A [1 ]
PAGE, M [1 ]
机构：
[1] UNIV LAVAL,FAC MED,DEPT BIOCHEM,ST FOY G1K 7P4,PQ,CANADA
来源：
JOURNAL OF CHROMATOGRAPHY A | 1994年 / 667卷 / 1-2期
关键词：
D O I：
10.1016/0021-9673(94)89060-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Human recombinant tumor necrosis factor alpha was purified in a single step to about 95% purity from Escherichia coli lysate by chromatography on hydroxyapatite. The last traces of contaminants were removed by fast protein liquid chromatography on a Mono Q column. The final product was found to be pure by gel electrophoresis with silver staining. A molecular mass of approximately 17 000 and a specific activity of 4.3 . 10(6) U/mg after a single purification step were found.
引用
收藏
页码：125 / 130
页数：6
相关论文
共 50 条
  • [1] INVIVO EFFECTS OF RECOMBINANT TUMOR-NECROSIS-FACTOR-ALPHA
    REMICK, DG
    KUNKEL, RG
    LARRICK, JW
    KUNKEL, SL
    FEDERATION PROCEEDINGS, 1987, 46 (04) : 1199 - 1199
  • [2] RAPID PURIFICATION OF RECOMBINANT HUMAN TUMOR-NECROSIS-FACTOR-BETA
    LOH, KC
    YAO, ZJ
    YAP, MGS
    CHUNG, MCM
    PROTEIN EXPRESSION AND PURIFICATION, 1994, 5 (01) : 70 - 75
  • [3] POTENTIATION OF PHOTODYNAMIC THERAPY IN MICE WITH RECOMBINANT HUMAN TUMOR-NECROSIS-FACTOR-ALPHA
    BELLNIER, DA
    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1991, 8 (02) : 203 - 210
  • [4] EFFECT OF INTRAPERITONEAL RECOMBINANT HUMAN TUMOR-NECROSIS-FACTOR-ALPHA ON MALIGNANT ASCITES
    RATH, U
    KAUFMANN, M
    SCHMID, H
    HOFMANN, J
    WIEDENMANN, B
    KIST, A
    KEMPENI, J
    SCHLICK, E
    BASTERT, G
    KOMMERELL, B
    MANNEL, D
    EUROPEAN JOURNAL OF CANCER, 1991, 27 (02) : 121 - 125
  • [5] Preparation and purification of pegylated recombinant human tumor necrosis factor alpha
    Jiang, YY
    Hong, MH
    Pei, YY
    CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2006, 34 (04) : 565 - 568
  • [6] ROLE OF TISSUE FACTOR IN THE ANTITUMOR EFFECT OF RECOMBINANT HUMAN TUMOR-NECROSIS-FACTOR-ALPHA IN MICE
    NISHIGAKI, F
    MIYAYASU, K
    TSUJIMOTO, S
    MANDA, T
    SHIMOMURA, K
    ANTICANCER RESEARCH, 1994, 14 (6B) : 2573 - 2576
  • [7] TUMOR-NECROSIS-FACTOR-ALPHA
    ROCK, CS
    LOWRY, SF
    JOURNAL OF SURGICAL RESEARCH, 1991, 51 (05) : 434 - 445
  • [8] RECOMBINANT HUMAN TUMOR-NECROSIS-FACTOR-ALPHA TRIGGERS INTRACELLULAR CALCIUM OSCILLATIONS IN HUMAN NEUTROPHILS
    SCHUMANN, MA
    RAFFIN, TA
    FASEB JOURNAL, 1992, 6 (01): : A124 - A124
  • [9] INTRAPLEURAL APPLICATION OF RECOMBINANT HUMAN TUMOR-NECROSIS-FACTOR-ALPHA IN MALIGNANT PLEURAL EFFUSIONS
    SCHONIG, T
    SCHMID, H
    KAUFMANN, M
    BASTERT, G
    ARCHIVES OF GYNECOLOGY AND OBSTETRICS, 1991, 250 (1-4) : 288 - 289
  • [10] SYNERGISTIC CYTOTOXICITY OF HUMAN RECOMBINANT TUMOR-NECROSIS-FACTOR-ALPHA COMBINED WITH MICROTUBULE EFFECTORS
    BAUMGART, J
    SCHLOTT, B
    SUEHNEL, J
    VATER, W
    SCHULZE, W
    BEHNKE, D
    JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, 1991, 117 (03) : 239 - 243
12345