IMMUNOCYTOCHEMICAL DEMONSTRATION OF NERVE GROWTH-FACTOR RECEPTOR (NGF-R) IN DEVELOPING HUMAN FETAL TEETH

被引:1
|
作者
CHRISTENSEN, LR
MOLLGARD, K
KJAER, I
JANAS, MS
机构
[1] UNIV COPENHAGEN,PANUM INST,DEPT MED ANAT A,DK-2200 COPENHAGEN,DENMARK
[2] UNIV COPENHAGEN,PANUM INST,CTR NEUROSCI,DK-2200 COPENHAGEN,DENMARK
来源
ANATOMY AND EMBRYOLOGY | 1993年 / 188卷 / 03期
关键词
HUMAN; FETAL; ODONTOGENESIS; NERVE GROWTH FACTOR RECEPTOR; NERVE GROWTH FACTOR;
D O I
暂无
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
Evidence is accumulating that nerve growth factor receptor (NGF-R or p75(NGFR)) can mediate cell growth and differentiation of non-neuronal cells. NGF-R expression was studied in developing teeth of human embryos and fetuses between the 6th and 18th weeks of gestation, using a monoclonal mouse-anti-human NGF-R antibody. In contrast to earlier findings in rodents, the NGF-R expression of the human dental papilla was found to be transient. NGF-R was present in the condensing ecto-mesenchymal cells of the dental papilla in the early cap stage tooth germ. In later developmental stages, a shift of the NGF-R expression from the papilla to the cytoplasmic membrane of the inner enamel epithelium (IEE) was demonstrated. As in rodent odontogenesis, the NGF-R immunoreactivity of the IEE remained until the odontoblasts started secretion of predentinal matrix in the late bell state. The mitotic activity in the IEE was detected by an antibody against proliferating cell nuclear antigen (PCNA) and showed that the NGFR expression of the IEE decreased as the cell proliferation ceased. We propose that NGF-R may, be involved in differentiational and/or proliferative events of human odontogenesis.
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页码:247 / 255
页数:9
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