Molecular typing of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from Shiraz teaching hospitals by PCR-RFLP of coagulase gene

被引：1
|
作者：
Khoshkharam-Roodmajani, Hossein [1 ,2 ]
Sarvari, Jamal [1 ]
Bazargani, Abdollah [1 ]
Kandekar-Ghahraman, Mohammad Reza [1 ]
Nazari-Alam, Ali [1 ]
Motamedifar, Mohammad [1 ,3 ]
机构：
[1] Shiraz Univ Med Sci, Fac Med, Dept Bacteriol & Virol, Shiraz, Iran
[2] Shiraz Univ Med Sci, Student Res Comm, Shiraz, Iran
[3] Shiraz Univ Med Sci, Shiraz HIV AIDS Res Ctr, Shiraz, Iran
来源：
IRANIAN JOURNAL OF MICROBIOLOGY | 2014年 / 6卷 / 04期
关键词：
Antibiotic resistance; MRSA; PCR; RFLP; Coagulase gene;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Background and Objectives: To investigate coagulase gene polymorphisms of MRSA and MSSA isolates from Shiraz teaching hospitals from 2011 to 2012. Materials and Methods: A total of 302 isolates of Staphylococcus aureus were collected from clinical specimens in three major teaching hospitals and confirmed on the basis of morphological characteristics and biochemical tests. The isolates were subjected to molecular typing on the basis of coagulase enzyme gene polymorphism by PCR-RFLP. Results: There were 27 and 28 different RFLP patterns for AluI and HaeIII restriction enzymes respectively. This study showed that the discriminatory power of coagulase gene typing by Hae III enzyme was more than that of Alu I enzyme. Conclusion: PCR-RFLP method is rapid, reproducible, simple and efficient for typing Staphylococcus aureus isolated from clinical specimens. This study showed that Hae III discriminatory power is better than Alu I for typing Staphylococcus aureus isolates.
引用
收藏
页码：246 / 252
页数：7
相关论文
共 50 条
  • [41] Molecular characterization of methicillin-resistant Staphylococcus aureus isolated from tertiary care hospitals
    Asghar, Atif H.
    PAKISTAN JOURNAL OF MEDICAL SCIENCES, 2014, 30 (04) : 698 - 702
  • [42] PCR-RFLP by AluI for coa gene of methicillin-resistant Staphylococcus aureus (MRSA) isolated from burn wounds, pneumonia and otitis media
    Chmagh, Aalaa A.
    Abd Al-Abbas, Munaff J.
    GENE REPORTS, 2019, 15
  • [43] Molecular typing of methicillin-resistant Staphylococcus aureus isolated in a Bahrain Hospital
    Udo, Edet E.
    Panigrahi, D.
    Jamsheer, A. E.
    MEDICAL PRINCIPLES AND PRACTICE, 2008, 17 (04) : 308 - 314
  • [44] Environment surface sampling in 33 Washington State fire stations for methicillin-resistant and methicillin-susceptible Staphylococcus aureus
    Roberts, Marilyn C.
    No, David B.
    AMERICAN JOURNAL OF INFECTION CONTROL, 2014, 42 (06) : 591 - 596
  • [45] The Detection and Differentiation of Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Endocarditis by Using the BD GeneOhm StaphSR Assay
    Frey, Amy B.
    Wilson, Deborah A.
    LaSalvia, Margaret M.
    Tan, Carmela D.
    Rodriguez, E. Rene
    Shrestha, Nabin K.
    Hall, Gerri S.
    Procop, Gary W.
    AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 2011, 136 (05) : 686 - 689
  • [46] Good Performance of the SpectraCellRA System for Typing of Methicillin-Resistant Staphylococcus aureus Isolates
    te Witt, R.
    Vaessen, N.
    Melles, D. C.
    Lekkerkerk, W. S. N.
    van der Zwaan, E. A. E.
    Zandijk, W. H. A.
    Severin, J. A.
    Vos, M. C.
    JOURNAL OF CLINICAL MICROBIOLOGY, 2013, 51 (05) : 1434 - 1438
  • [47] Evaluation of spa Typing for the Classification of Clinical Methicillin-Resistant Staphylococcus aureus Isolates
    Furuya, Daisuke
    Tsuji, Naoki
    Kuribayashi, Kageaki
    Tanaka, Maki
    Hosono, Yukie
    Uehara, Nobuyuki
    Watanabe, Naoki
    JAPANESE JOURNAL OF INFECTIOUS DISEASES, 2010, 63 (05) : 364 - 367
  • [48] The epidemiology of methicillin-susceptible and methicillin-resistant Staphylococcus aureus in a neonatal intensive care unit, 2000-2007
    Carey, A. J.
    Duchon, J.
    Della-Latta, P.
    Saiman, L.
    JOURNAL OF PERINATOLOGY, 2010, 30 (02) : 135 - 139
  • [49] Molecular characterization of methicillin-resistant Staphylococcus aureus bloodstream isolates from Croatia
    Budimir, A
    Deurenberg, RH
    Plecko, V
    Vink, C
    Kalenic, S
    Stobberingh, EE
    JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2006, 57 (02) : 331 - 334
  • [50] COMPARATIVE-STUDY OF THE CELLULAR FATTY-ACIDS OF METHICILLIN-RESISTANT AND METHICILLIN-SUSCEPTIBLE STAPHYLOCOCCUS-AUREUS
    ASAI, S
    NODA, M
    YAMAMURA, M
    HOZUMI, Y
    TAKASE, I
    NITTA, H
    SATO, M
    NAMIKAWA, I
    APMIS, 1993, 101 (10) : 753 - 761
12345