ARABINAN DEGRADING ENZYMES FROM ASPERGILLUS-NIDULANS - INDUCTION AND PURIFICATION

被引：42

作者：

RAMON, D

VANDERVEEN, P

VISSER, J

机构：

[1] WAGENINGEN UNIV AGR,DEPT GENET,MOLEC GENET SECT,DREYENLAAN 2,6703 HA WAGENINGEN,NETHERLANDS

[2] CSIC,INST AGROQUIM & TECNOL ALIMENTOS,UNIDAD BIOINGN,E-46010 VALENCIA,SPAIN

来源：

FEMS MICROBIOLOGY LETTERS | 1993年 / 113卷 / 01期

关键词：

ARABINASES; ARABINOFURANOSIDASES; ASPERGILLUS-NIDULANS;

D O I：

10.1016/0378-1097(93)90092-G

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The presence in Aspergillus nidulans of two enzymes related to the Aspergillus niger endo-arabinase and alpha-L-arabinofuranosidase B has been established using antibodies against the purified A. niger enzymes. Moreover, the absence of an equivalent in A. nidulans to the alpha-L-arabinofuranosidase A of A. niger has been confirmed both at the protein and at the DNA level. Both A. nidulans arabinases have been purified and physico-chemically and kinetically characterized. They have a much higher temperature optimum than the corresponding A. niger enzymes. The pattern of induction has been studied on media containing different carbon sources showing an important role of L-arabitol in the induction of these enzymes.

引用

页码：15 / 22

页数：8

共 19 条

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