INTERSUBUNIT LOCATION OF THE ACTIVE-SITE OF MAMMALIAN ORNITHINE DECARBOXYLASE AS DETERMINED BY HYBRIDIZATION OF SITE-DIRECTED MUTANTS

被引:74
|
作者
TOBIAS, KE [1 ]
KAHANA, C [1 ]
机构
[1] WEIZMANN INST SCI,DEPT MOLEC GENET & VIROL,IL-76100 REHOVOT,ISRAEL
关键词
D O I
10.1021/bi00073a017
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The active form of mammalian ornithine decarboxylase (ODC) is a homodimer consisting of two monomer subunits of 53 kDa each. We have used in vitro hybridization of two different catalytically inactive mutants of ODC to determine whether in the wild-type enzyme each monomer contains an independent active site or whether the active sites are shared at the interfaces between the two subunits. Two distinct mutants were obtained using oligonucleotide-directed mutagenesis: In one, cysteine-360, the major alpha-(difluoromethyl)ornithine (alpha-DFMO, a suicide inhibitor of ODC) binding site was converted to alanine. In the other, lysine-69, the pyridoxal 5'-phosphate (PLP, the cofactor of ODC) binding residue was converted toalanine. Expression of each mutant, in vitro, in reticulocyte lysate translation mix, results in the production of a completely inactive enzyme. In contrast, their coexpression restores enzymatic activity to about 25% of the wild-type enzyme. Moreover, coexpression of wild-type subunits with monomers containing both inactivating mutations reduced their activity to about 25%, while their coexpression with monomers that contain a single inactivating mutation reduced the activity to 50%. Cross-linking analysis has demonstrated that activity restoration and repression are both fully correlated with the formation of heterodimers between mutant subunits and between mutant and wild-type subunits, respectively. We therefore conclude that the active site of ODC is formed at the interface of the two monomers through the interaction of the cysteine-360-containing region of one monomer subunit with the region that contains lysine-69 of the other subunit.
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页码:5842 / 5847
页数:6
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