CALLUS DEVELOPMENT AND PLANT-REGENERATION FROM DIFFERENT EXPLANTS OF 6 SPECIES OF SUNFLOWER (HELIANTHUS L)

被引：20
作者：
PUNIA, MS [1 ]
BOHOROVA, NE [1 ]
机构：
[1] BULGARIAN ACAD SCI, INST GENET, BU-1113 SOFIA, BULGARIA
来源：
PLANT SCIENCE | 1992年 / 87卷 / 01期
关键词：
HELIANTHUS L; SUNFLOWER; CALLUS; REGENERATION; PLANTLETS; ORGANOGENESIS; EMBRYOGENESIS;
D O I：
10.1016/0168-9452(92)90195-R
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Four explants, stem, leaf, bud and cotyledons of six wild species of Helianthus viz., H. nuttallii, H. mollis, H. debilis, H. divaricatus, H. maximilliani and H. praecox, were cultured on different media for callus development. Calli were regenerated on Murashige-Skoog (MS) medium supplemented with 1.0 mg/l 6-benzylamino purine (BAP), 0.1 mg/l gibberellic acid (GA3), 500 mg/l casamino acids (CA) and 40 mg/l adenine sulphate (AS) (R medium). Regenerated plantlets were grown on MSD4 medium to form complete plants. The plants were rooted on MS basal hormone free and BGS media.
引用
收藏
页码：79 / 83
页数：5
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