CALLUS DEVELOPMENT AND PLANT-REGENERATION FROM DIFFERENT EXPLANTS OF 6 SPECIES OF SUNFLOWER (HELIANTHUS L)

被引：20

作者：

PUNIA, MS ^{[1
]}

BOHOROVA, NE ^{[1
]}

机构：

[1] BULGARIAN ACAD SCI, INST GENET, BU-1113 SOFIA, BULGARIA

来源：

PLANT SCIENCE | 1992年 / 87卷 / 01期

关键词：

HELIANTHUS L; SUNFLOWER; CALLUS; REGENERATION; PLANTLETS; ORGANOGENESIS; EMBRYOGENESIS;

D O I：

10.1016/0168-9452(92)90195-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Four explants, stem, leaf, bud and cotyledons of six wild species of Helianthus viz., H. nuttallii, H. mollis, H. debilis, H. divaricatus, H. maximilliani and H. praecox, were cultured on different media for callus development. Calli were regenerated on Murashige-Skoog (MS) medium supplemented with 1.0 mg/l 6-benzylamino purine (BAP), 0.1 mg/l gibberellic acid (GA3), 500 mg/l casamino acids (CA) and 40 mg/l adenine sulphate (AS) (R medium). Regenerated plantlets were grown on MSD4 medium to form complete plants. The plants were rooted on MS basal hormone free and BGS media.

引用

页码：79 / 83

页数：5

共 14 条

[1]

BOHOROVA N, 1985, Z PFLANZENZUCHT, V95, P35

[2] ISOLATION, CULTURE AND CALLUS REGENERATION OF PROTOPLASTS OF WILD AND CULTIVATED HELIANTHUS SPECIES [J].

BOHOROVA, NE ;

COCKING, EC ;

POWER, JB .

PLANT CELL REPORTS, 1986, 5 (04) :256-258

[3] THE RESPONSE OF NORMAL PLANT TISSUES AND OF CROWN GALL TUMOR TISSUES TO SYNTHETIC GROWTH HORMONES [J].

DEROPP, RS .

AMERICAN JOURNAL OF BOTANY, 1947, 34 (02) :53-62

[4]

ESPINASSE A, 1988, 12TH P INT SUNFL C N, P309

[5] GENETIC-ENGINEERING OF SUNFLOWER (HELIANTHUS-ANNUUS L) [J].

EVERETT, NP ;

ROBINSON, KEP ;

MASCARENHAS, D .

BIO-TECHNOLOGY, 1987, 5 (11) :1201-1204

[6] CALLUS INDUCTION AND SHOOT REGENERATION IN SUNFLOWER (HELIANTHUS-ANNUUS L) [J].

GRECO, B ;

TANZARELLA, OA ;

CARROZZO, G ;

BLANCO, A .

PLANT SCIENCE LETTERS, 1984, 36 (01) :73-77

[7]

HARTMAN C, 1988, 12TH P INT SUNFL C N, P374

[8] THE INFLUENCE OF THE COMPOSITION OF THE MEDIUM ON GROWTH INVITRO OF EXCISED TOBACCO AND SUNFLOWER TISSUE CULTURES [J].

HILDEBRANDT, AC ;

RIKER, AJ ;

DUGGAR, BM .

AMERICAN JOURNAL OF BOTANY, 1946, 33 (07) :591-597

[9] PLANTLET FORMATION FROM CALLUS AND SHOOT-TIP CULTURE OF HELIANTHUS-ANNUUS (L) [J].

LUPI, MC ;

BENNICI, A ;

LOCCI, F ;

GENNAI, D .

PLANT CELL TISSUE AND ORGAN CULTURE, 1987, 11 (01) :47-55

[10] A SYSTEM FOR ROUTINE PLANTLET REGENERATION OF SUNFLOWER (HELIANTHUS-ANNUUS L) FROM IMMATURE EMBRYO-DERIVED CALLUS [J].

MCCANN, AW ;

COOLEY, G ;

VANDRESER, J .

PLANT CELL TISSUE AND ORGAN CULTURE, 1988, 14 (02) :103-110

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