EFFECTS OF NA+ ON THE PERSISTENCE LENGTH AND EXCLUDED VOLUME OF T7-BACTERIOPHAGE DNA

被引：72

作者：

SOBEL, ES

HARPST, JA

机构：

[1] Department of Biochemistry, Case Western Reserve University, Cleveland, Ohio

[2] Department of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina

来源：

BIOPOLYMERS | 1991年 / 31卷 / 13期

关键词：

D O I：

10.1002/bip.360311311

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Total intensity, Rayleigh light scattering has been used to measure the rms radius, second virial coefficient, persistence length, and excluded volume of homogeneous T7 bacteriophage DNA as a function of Na+ concentration (0.005 to 3.0 M). All parameters decrease sharply as [Na+] increases, and tend to level off at high Na+. The variation of persistence length with [Na+] is consistent with predictions from counterion condensation theory.

引用

页码：1559 / 1564

页数：6

共 43 条

[1] PRACTICAL PROCEDURES FOR PURIFICATION OF BACTERIAL VIRUSES [J].