LOCALIZATION OF ISOPENICILLIN N SYNTHASE IN PENICILLIUM-CHRYSOGENUM PQ-96

被引：15
作者：
KURZATKOWSKI, W
PALISSA, H
VANLIEMPT, H
VONDOHREN, H
KLEINKAUF, H
WOLF, WP
KURYLOWICZ, W
机构：
[1] INST BIOCHEM & MOLEC BIOL,W-1000 BERLIN 10,GERMANY
[2] ROBERT KOCH INST,FED HLTH OFF,W-1000 BERLIN 65,GERMANY
来源：
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1991年 / 35卷 / 04期
关键词：
D O I：
10.1007/BF00169760
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
For immunoelectron microscopic localization of isopenicillin N synthase (IPNS), glutaraldehyde-fixed mycelium of Penicillium chrysogenum PQ-96 was dehydrated by progressive lowering of the temperature and embedded into Lowicryl K4M at -35-degrees-C. This procedure resulted in good structural preservation such that the method of on-section labelling using antibody to IPNS from Cephalosporium acremonium CO 278 with the indirect antibody-gold technique could be successfully applied. By this method IPNS was localized in vesicular compartments belonging probably to the Golgi body and in the cell wall.
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收藏
页码：517 / 520
页数：4
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