OCCURRENCE OF FATTY-ACID EPOXIDE HYDROLASES IN SOYBEAN (GLYCINE-MAX) - PURIFICATION AND CHARACTERIZATION OF THE SOLUBLE FORM

被引：61

作者：

BLEE, E ^{[1
]}

SCHUBER, F ^{[1
]}

机构：

[1] FAC PHARM ILLKIRCH, CHIM BIOORGAN LAB, CNRS, URA 1386, F-67400 ILLKIRCH GRAFFENS, FRANCE

来源：

BIOCHEMICAL JOURNAL | 1992年 / 282卷

关键词：

D O I：

10.1042/bj2820711

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Epoxide hydrolases catalysing the hydration of cis-9,10-epoxystearate into threo-9,10-dihydroxystearate have been detected in soybean (Glycine max) seedlings. The major activity was found in the cytosol, a minor fraction being strongly associated with microsomes. The soluble enzyme, which was purified to apparent homogeneity by (NH4)2SO4 fractionation, hydrophobic, DEAE- and gel-filtration chromatographies, has a molecular mass of 64 kDa and a pI of 5.4.

引用

页码：711 / 714

页数：4

共 19 条

[1]

BLEE E, 1990, J BIOL CHEM, V265, P12887

[2] MECHANISM OF S-OXIDATION REACTIONS CATALYZED BY A SOYBEAN HYDROPEROXIDE-DEPENDENT OXYGENASE [J].

BLEE, E ;

SCHUBER, F .

BIOCHEMISTRY, 1989, 28 (12) :4962-4967

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4] BIOSYNTHESIS OF HYDROXYFATTY ACID POLYMERS - ENZYMATIC EPOXIDATION OF 18-HYDROXYOLEIC ACID TO 18-HYDROXY-CIS-9,10-EPOXYSTEARIC ACID BY A PARTICULATE PREPARATION FROM SPINACH (SPINACIA-OLERACEA) [J].

CROTEAU, R ;

KOLATTUKUDY, PE .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1975, 170 (01) :61-72

[5]

FERSHT A, 1977, ENZYME STRUCTURE MEC, pCH3

[6] HYDRATION OF CIS-EPOXYMETHYL AND TRANS-EPOXYMETHYL STEARATES BY THE CYTOSOLIC EPOXIDE HYDRASE OF MOUSE-LIVER [J].

GILL, SS ;

HAMMOCK, BD .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1979, 89 (03) :965-971

[7]

GUENTHNER TM, 1983, J BIOL CHEM, V258, P5054

[8]

Holloway P. J., 1982, The plant cuticle, P45

[9] DEFENSE-MECHANISM OF THE RICE PLANT AGAINST RICE BLAST DISEASE [J].

KATO, T ;

YAMAGUCHI, Y ;

UYEHARA, T ;

YOKOYAMA, T ;

NAMAI, T ;

YAMANAKA, S .

NATURWISSENSCHAFTEN, 1983, 70 (04) :200-201

[10] STRUCTURE, BIOSYNTHESIS, AND BIODEGRADATION OF CUTIN AND SUBERIN [J].

KOLATTUKUDY, PE .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1981, 32 :539-567

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