CLONING AND SEQUENCE-ANALYSIS OF A CDNA FOR HUMAN GLYCOSYLASPARAGINASE - A SINGLE GENE ENCODES THE SUBUNITS OF THIS LYSOSOMAL AMIDASE

被引：45

作者：

FISHER, KJ

TOLLERSRUD, OK

ARONSON, NN

机构：

[1] Department of Molecular and Cell Biology, Althouse Laboratory, The Pennsylvania State University, University Park

来源：

FEBS LETTERS | 1990年 / 269卷 / 02期

关键词：

Aspartylglucosaminuria; cDNA; Glycoprotein degradation; Glycosylasparaginase; Lysosome;

D O I：

10.1016/0014-5793(90)81211-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated a full-length cDNA (HPAsn.6) for human placenta glycosylasparaginase using a 221-bp PCR amplified fragment containing rat liver asparaginase gene sequences. The deduced amino acid sequence from the human clone showed sequence identity to both the α and β subunits of the rat enzyme. The human enzyme is encoded as a 34.6 kDa polypeptide that is post-translationally processed to generate two subunits of approx. 19.5 (α) and 15 (β) kDa. A charge enriched region is present at the predicted site where cleavage occurs. Using polyclonal antibodies against the α and β subunits of rat liver asparaginase, we have shown that the human enzyme is similar in structure to the rat enzyme. © 1990.

引用

页码：440 / 444

页数：5

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