MOLECULAR DETECTION OF CANINE DISTEMPER VIRUS IN CLINICAL CASES FROM UNVACCINATED DOMESTIC DOGS AND ASSESSMENT OF RISK FACTORS

被引：1

作者：

Soto R, Andrea ^{[1
]}

Luna E., Luis R. ^{[1
]}

Rosadio A, Raul ^{[1
]}

Maturrano H, Lenin ^{[2
]}

机构：

[1] Univ Nacl Mayor San Marcos, Fac Med Vet, Lab Microbiol & Parasitol Vet, Lima, Peru

[2] Univ Nacl Mayor San Marcos, Fac Med Vet, Lab Zootecnia & Prod Agr, Lima, Peru

来源：

REVISTA DE INVESTIGACIONES VETERINARIAS DEL PERU | 2018年 / 29卷 / 03期

关键词：

canine distemper; molecular detection; nucleoprotein; PCR;

D O I：

10.15381/rivep.v29i3.14744

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The canine distemper (CD) is a viral infection caused by a Morbillivirus of the family Paramyxoviridae. It can generate digestive, respiratory, and nervous symptomatology, depending on the infecting strain. This study was designed to detect the CD virus through the RT-PCR technique and perform the analysis of the data collected to have a current view of the behavior of this virus in Lima, Peru. Whole blood samples from 52 unvaccinated canines with clinical signs compatible with canine distemper were collected between June 2012 and January 2015. The nucleoprotein of 287 bp was successfully amplified in 32.7% (17/52) of the samples analyzed. In addition, it was found a greater detection of the virus, but not significative, in individuals that manifested systemic clinical signs (respiratory and digestive signs vs nervous) and within the age range of 1.5-4 months.

引用

页码：964 / 971

页数：8

共 25 条

[11] Detection of canine distemper virus nucleoprotein RNA by reverse transcription-PCR using serum, whole blood, and cerebrospinal fluid from dogs with distemper [J].

Frisk, AL ;

König, M ;

Moritz, A ;

Baumgärtner, W .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (11) :3634-3643

[12] Identification of a new genotype of canine distemper virus circulating in America [J].

Gamiz, Cesar ;

Martella, Vito ;

Ulloa, Raul ;

Fajardo, Raul ;

Quijano-Hernandez, Israel ;

Martinez, Simon .

VETERINARY RESEARCH COMMUNICATIONS, 2011, 35 (06) :381-390

[13]

Greene CE, 2012, INFECT DIS DOG CAT, V4th

[14] Natural distemper in vaccinated and unvaccinated dogs in Warsaw [J].

Józwik, A ;

Frymus, T .

JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH, 2002, 49 (09) :413-414

[15]

Lorenzana L., 2008, ACTUALIZACION TERAPE

[16] Canine distemper virus [J].

Martella, Vito ;

Elia, Gabrielle ;

Buonavoglia, Canio .

VETERINARY CLINICS OF NORTH AMERICA-SMALL ANIMAL PRACTICE, 2008, 38 (04) :787-+

[17] Genotypes of canine distemper virus determined by analysis of the hemagglutinin genes of recent isolates from dogs in Japan [J].

Mochizuki, M ;

Hashimoto, M ;

Hagiwara, S ;

Yoshida, Y ;

Ishiguro, S .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (09) :2936-2942

[18]

Panzera Y, 2014, BR J VIROL, V1, P48

[19] Evidence of two co-circulating genetic lineages of canine distemper virus in South America [J].

Panzera, Yanina ;

Gallo Calderon, Marina ;

Sarute, Nicolas ;

Guasco, Soledad ;

Cardeillac, Arianne ;

Bonilla, Braulio ;

Hernandez, Martin ;

Francia, Lourdes ;

Bedo, Gabriela ;

La Torre, Jose ;

Perez, Ruben .

VIRUS RESEARCH, 2012, 163 (01) :401-404

[20] Canine distemper virus uses both the anterograde and the hematogenous pathway for neuroinvasion [J].

Rudd, Penny A. ;

Cattaneo, Roberto ;

von Messling, Veronika .

JOURNAL OF VIROLOGY, 2006, 80 (19) :9361-9370

← 1 2 3 →