IMPROVED YIELD BY CRYOEFFECT IN KINETICALLY CONTROLLED PEPTIDE-SYNTHESIS CATALYZED WITH ALCALASE

被引：7

作者：

CHEN, ST ^{[1
]}

CHEN, SY ^{[1
]}

KAO, CL ^{[1
]}

WANG, KT ^{[1
]}

机构：

[1] NATL TAIWAN UNIV,DEPT CHEM,TAIPEI,TAIWAN

来源：

BIOTECHNOLOGY LETTERS | 1994年 / 16卷 / 10期

关键词：

D O I：

10.1007/BF01022406

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The yields of a kinetically controlled enzymatic reaction catalyzed by alcalase in alcohols increased 15%similar to 40% when the reaction temperature was 5 degrees C instead of 25-30 degrees C. High yields of biologically active peptide intermediates were obtained from reaction at 5 degrees C.

引用

页码：1075 / 1080

页数：6

共 17 条

[1] ENZYMATIC CATALYSIS AND DYNAMICS IN LOW-WATER ENVIRONMENTS
AFFLECK, R
XU, ZF
SUZAWA, V
FOCHT, K
CLARK, DS
DORDICK, JS
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (03) : 1100 - 1104
[2] A SEARCH FOR PEPTIDE LIGASE - COSOLVENT-MEDIATED CONVERSION OF PROTEASES TO ESTERASES FOR IRREVERSIBLE SYNTHESIS OF PEPTIDES
BARBAS, CF
MATOS, JR
WEST, JB
WONG, CH
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1988, 110 (15) : 5162 - 5166
[3] BENDER ML, 1964, J AM CHEM SOC, V86, P3097
[4] KINETICALLY CONTROLLED PEPTIDE-BOND FORMATION IN ANHYDROUS ALCOHOL CATALYZED BY THE INDUSTRIAL PROTEASE ALCALASE
CHEN, ST
CHEN, SY
WANG, KT
[J]. JOURNAL OF ORGANIC CHEMISTRY, 1992, 57 (25) : 6960 - 6965
[5] DESIGNING ENZYMES FOR USE IN ORGANIC-SOLVENTS
DORDICK, JS
[J]. BIOTECHNOLOGY PROGRESS, 1992, 8 (04) : 259 - 267
[6] DEMONSTRATION OF ACYL-ENZYME MECHANISM FOR HYDROLYSIS OF PEPTIDES AND ANILIDES BY CHYMOTRYPSIN
FASTREZ, J
FERSHT, AR
[J]. BIOCHEMISTRY, 1973, 12 (11) : 2025 - 2034
[7] HOW CAN THE SOLVENT AFFECT ENZYME ENANTIOSELECTIVITY
FITZPATRICK, PA
KLIBANOV, AM
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (08) : 3166 - 3171
[8] ENZYME FUNCTION IN ORGANIC-SOLVENTS
GUPTA, MN
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 203 (1-2): : 25 - 32
[9] UNUSUAL SOLVENT EFFECT ON PROTEASE ACTIVITY AND EFFECTIVE OPTICAL RESOLUTION OF AMINO-ACIDS BY HYDROLYTIC REACTIONS IN ORGANIC-SOLVENTS
KISE, H
TOMIUCHI, Y
[J]. BIOTECHNOLOGY LETTERS, 1991, 13 (05) : 317 - 322
[10] KURT N, 1992, BIOTECH APPLIED BIOC, V16, P182

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