INTERLEUKIN-1-BETA (IL-1-BETA) INDUCES TRANSFORMING GROWTH FACTOR-BETA(1) (TGF-BETA(1)) PRODUCTION BY RAT AORTIC SMOOTH-MUSCLE CELLS

被引：37
作者：
YUE, TL
WANG, XK
OLSON, B
FEUERSTEIN, G
机构：
[1] Department of Cardiovascular Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 204卷 / 03期
关键词：
D O I：
10.1006/bbrc.1994.2588
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In cultured rat aortic smooth muscle cells (RASMCs) IL-1 beta induced TGF-beta(1) mRNA expression, which was concentration (10 pM-10 nM)- and time (2-48 h)-dependent, and sensitive to cycloheximide. The maximal increase in TGF-beta(1) mRNA was 4.5 times the basal level at 10 nM of IL-1 beta. A parallel increase In TGF-beta(1) protein in the culture medium of IL-1 beta-stimulated RASMCs was also observed. A detectable level of TGF-beta(1) was found 8 h after stimulation and a plateau was reached at 72 h with a maximal increase of 5-fold and was inhibited by IL-1 receptor antagonist (IL-1ra). Our data demonstrate for the first time that IL-1 beta is a potent inducer of TGF-beta(1) synthesis and release in vascular smooth muscle cells. (C) 1994 Academic Press, Inc.
引用
收藏
页码：1186 / 1192
页数：7
相关论文
共 16 条
[1]   EXPRESSION AND SECRETION OF TYPE-BETA TRANSFORMING GROWTH-FACTOR BY ACTIVATED HUMAN MACROPHAGES [J].
ASSOIAN, RK ;
FLEURDELYS, BE ;
STEVENSON, HC ;
MILLER, PJ ;
MADTES, DK ;
RAINES, EW ;
ROSS, R ;
SPORN, MB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (17) :6020-6024
[2]   TGF-BETA RECEPTORS AND ACTIONS [J].
ATTISANO, L ;
WRANA, JL ;
LOPEZCASILLAS, F ;
MASSAGUE, J .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1994, 1222 (01) :71-80
[3]   TRANSFORMING GROWTH-FACTOR TYPE-BETA SPECIFICALLY STIMULATES SYNTHESIS OF PROTEOGLYCAN IN HUMAN ADULT ARTERIAL SMOOTH-MUSCLE CELLS [J].
CHEN, JK ;
HOSHI, H ;
MCKEEHAN, WL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (15) :5287-5291
[4]  
CLINTON S, ARCH PATHOL LAB MED, V116, P1292
[5]  
DINARELLO CA, 1993, NEW ENGL J MED, V328, P106
[6]   ADULT HUMAN AORTIC SMOOTH-MUSCLE CELLS IN CULTURE PRODUCE ACTIVE TGF-BETA [J].
KIRSCHENLOHR, HL ;
METCALFE, JC ;
WEISSBERG, PL ;
GRAINGER, DJ .
AMERICAN JOURNAL OF PHYSIOLOGY, 1993, 265 (02) :C571-C576
[7]   BIFUNCTIONAL EFFECTS OF TRANSFORMING GROWTH-FACTOR-BETA ON MIGRATION OF CULTURED RAT AORTIC SMOOTH-MUSCLE CELLS [J].
KOYAMA, N ;
KOSHIKAWA, T ;
MORISAKI, N ;
SAITO, Y ;
YOSHIDA, S .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 169 (02) :725-729
[8]   PRODUCTION OF TRANSFORMING GROWTH FACTOR-BETA-1 DURING REPAIR OF ARTERIAL INJURY [J].
MAJESKY, MW ;
LINDNER, V ;
TWARDZIK, DR ;
SCHWARTZ, SM ;
REIDY, MA .
JOURNAL OF CLINICAL INVESTIGATION, 1991, 88 (03) :904-910
[9]   EXPRESSION OF TRANSFORMING GROWTH FACTOR-BETA-1 IS INCREASED IN HUMAN VASCULAR RESTENOSIS LESIONS [J].
NIKOL, S ;
ISNER, JM ;
PICKERING, JG ;
KEARNEY, M ;
LECLERC, G ;
WEIR, L .
JOURNAL OF CLINICAL INVESTIGATION, 1992, 90 (04) :1582-1592
[10]   CYTOKINES AND SMOOTH-MUSCLE CELLS IN ATHEROSCLEROSIS [J].
NILSSON, J .
CARDIOVASCULAR RESEARCH, 1993, 27 (07) :1184-1190
12