EFFECT OF EXOGENOUS GIBBERELLIC-ACID, ABSCISIC-ACID, AND BENZYLAMINOPURINE ON EPICOTYL DORMANCY OF CULTURED HERBACEOUS PEONY EMBRYOS

被引：17

作者：

BUCHHEIM, JAT ^{[1
]}

BURKHART, LF ^{[1
]}

MEYER, MM ^{[1
]}

机构：

[1] UNIV ILLINOIS,DEPT HORT,URBANA,IL 61801

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1994年 / 36卷 / 01期

关键词：

EMBRYO CULTURE; IMAGE ANALYSIS; PLANT GROWTH REGULATORS; SEED GERMINATION; TISSUE CULTURE;

D O I：

10.1007/BF00048313

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Epicotyl dormancy was broken in cultured peony (Paeonia lactiflora Pall.) embryos after topical application of agarose gels containing gibberellic acid, with optimum growth at 1.5 mM gibberellic acid. Addition of 100 muM abscisic acid to the medium resulted in complete inhibition of gibberellic acid-stimulated promotion of dormant epicotyls. Epicotyl dormancy was also broken in embryos by culture on media containing 1 or 10 muM benzylaminopurine. A1 highly significant increase in leaf number occurred when embryos were both cultured on medium containing benzylaminopurine and treated topically with gibberellic acid. Anatomical and morphological studies indicated that the increase in shoot growth was due to the development and growth of 1) buds formed at the cotyledonary node, 2) axillary buds, and 3) adventitious meristems originating from subepidermal parenchymatous tissue.

引用

页码：35 / 43

页数：9

共 35 条

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