SEX PRESELECTION IN SWINE - ALTERED SEX-RATIOS IN OFFSPRING FOLLOWING SURGICAL INSEMINATION OF FLOW SORTED X-BEARING AND Y-BEARING SPERM

被引：155

作者：

JOHNSON, LA ^{[1
]}

机构：

[1] USDA ARS,BELTSVILLE AGR RES CTR,PHYSIOL LAB,BELTSVILLE,MD 20705

来源：

REPRODUCTION IN DOMESTIC ANIMALS | 1991年 / 26卷 / 06期

关键词：

SEMEN; CELL SORTER; FLOW SORTING; DNA; BIRTH;

D O I：

10.1111/j.1439-0531.1991.tb01546.x

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Flow cytometric sorting of X and Y chromosome-bearing sperm into separate populations, followed by surgical insemination into the isthmus of the oviduct of mature gilts, produced litters with phenotypic sex ratios skewed in the direction of male or female according to the predicted sex of the sperm population inseminated. The skewing of the sex ratio of the offspring was predicted from flow cytometric DNA reanalysis of flow sorted sperm populations. Gilts inseminated with intact, viable flow-sorted X-bearing sperm produced litters of offspring that were 74% female (P < .01). Gilts inseminated with intact, viable flow-sorted Y-bearing sperm gave offspring that were 68% male (P < .04). These results validate the use of DNA as a marker for pre-selecting the sex of offspring when used in conjunction with flow cytometric sorting of sperm.

引用

页码：309 / 314

页数：6

共 9 条

[1] FLOW SORTING OF X-CHROMOSOME-BEARING AND Y-CHROMOSOME-BEARING SPERMATOZOA INTO 2 POPULATIONS [J].

JOHNSON, LA ;

FLOOK, JP ;

LOOK, MV ;

PINKEL, D .

GAMETE RESEARCH, 1987, 16 (01) :1-9

[2]

JOHNSON LA, 1988, ZUCHTHYGIENE, V23, P49

[3] MODIFICATION OF A LASER-BASED FLOW CYTOMETER FOR HIGH-RESOLUTION DNA ANALYSIS OF MAMMALIAN SPERMATOZOA [J].

JOHNSON, LA ;

PINKEL, D .

CYTOMETRY, 1986, 7 (03) :268-273

[4] SEX PRESELECTION IN RABBITS - LIVE BIRTHS FROM X-SPERM AND Y-SPERM SEPARATED BY DNA AND CELL SORTING [J].

JOHNSON, LA ;

FLOOK, JP ;

HAWK, HW .

BIOLOGY OF REPRODUCTION, 1989, 41 (02) :199-203

[5] FLOW SORTING OF X-CHROMOSOME-BEARING AND Y-CHROMOSOME-BEARING MAMMALIAN SPERM - ACTIVATION AND PRONUCLEAR DEVELOPMENT OF SORTED BULL, BOAR, AND RAM SPERM MICROINJECTED INTO HAMSTER OOCYTES [J].

JOHNSON, LA ;

CLARKE, RN .

GAMETE RESEARCH, 1988, 21 (04) :335-343

[6]

JOHNSON LA, 1973, J ANIM SCI, V37, P317

[7] FLOW-CYTOMETRY OF X-CHROMOSOME-BEARING AND Y-CHROMOSOME-BEARING SPERM FOR DNA USING AN IMPROVED PREPARATION METHOD AND STAINING WITH HOECHST-33342 [J].

JOHNSON, LA ;

FLOOK, JP ;

LOOK, MV .

GAMETE RESEARCH, 1987, 17 (03) :203-212

[8]

JOHNSON LA, 1986, 10 BELTS S AGR RES, P121

[9]

1985, SAS STAT GUIDE PERSO

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