WHOLE-BLOOD SUPEROXIDE ANION GENERATION AND EFFICIENCY OF SOME ERYTHROCYTE ANTIOXIDANT SYSTEMS DURING RECOMBINANT-HUMAN-ERYTHROPOIETIN THERAPY OF UREMIC ANEMIA

被引：10
作者：
LUCIAK, M
PAWLICKI, L
KEDZIORA, J
TRZNADEL, K
BLASZCZYK, J
BUCZYNSKI, A
机构：
[1] 2nd Department of Internal Medicine, Institute of Internal Medicine, WAM, 90-001 łódź 1
[2] Department of Physiology, Institute of Fundamental Sciences, WAM, 90-647 Łódź
来源：
FREE RADICAL BIOLOGY AND MEDICINE | 1991年 / 10卷 / 06期
关键词：
SUPEROXIDE ANION; SUPEROXIDE DISMUTASE; GLUTATHIONE PEROXIDASE; MALONYLDIALDEHYDE; ERYTHROPOIETIN; UREMIC ANEMIA;
D O I：
10.1016/0891-5849(91)90048-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Six chronic uremic patients on regular hemodialysis treatment were given recombinant human erythropoietin (r-huEPO) in a dose of 50 U/kg of body weight intravenously thrice weekly for 14 weeks. Following r-huEPO therapy, unstimulated whole blood superoxide anion (O2) generation did not change significantly, while opsonized zymosan-stimulated whole blood O2 generation increased. At the same time, erythrocyte superoxide dismutase and, in particular, glutathione peroxidase activities were found to be reducing with concomitant lowering of erythrocyte malonyldialdehyde (MDA) concentrations and increase in plasma MDA concentrations.
引用
收藏
页码：397 / 401
页数：5
相关论文
共 39 条
[1]  
Caro, Brown, Miller, Murray, Erslev, Erythropoietin levels in uremic nephric and anephric patients, J. Lab. Clin. Med., 93, pp. 449-458, (1979)
[2]  
Eschbach, Mladenovic, Garcia, Wahl, Adamson, The anemia of chronic renal failure in sheep: response to erythropoietin-rich plasma in vivo, J. Clin. Invest., 73, pp. 434-441, (1984)
[3]  
Eschbach, Adamson, Modern aspects of the pathophysiology of renal anemia, Contr. Nephrol., 66, pp. 63-70, (1988)
[4]  
Yawata, Jacob, Abnormal red cell metabolism in patients with chronic uremia: nature of the defect and its persistence despite adequate hemodialysis, Blood, 45, pp. 231-239, (1975)
[5]  
Eaton, Leida, Hemolysis in chronic renal failure, Semin. Nephrol., 5, pp. 133-139, (1985)
[6]  
Yawata, Howe, Jacob, Abnormal red cell metabolism causing hemolysis in uremia. A defect potentiated by tap water hemodialysis, Ann. Intern. Med., 79, pp. 362-367, (1973)
[7]  
De Leeuw, Shapiro, Lowenstein, Drug-induced hemolytic anemia, Ann. Intern. Med., 58, pp. 592-607, (1963)
[8]  
Chauhan, Gupta, Nampoothiri, Singhal, Chugh, Nair, Determination of erythrocyte superoxide dismutase, catalase, glucose-6-phosphate dehydrogenase, reduced glutathione and malonyldialdehyde in uremia, Clin. Chim. Acta, 123, pp. 153-159, (1982)
[9]  
Giardini, Taccone-Galluci, Lubrano, Ricciardi-Tenore, Bandino, Silvi, Ruberto, Casciani, Evidence of red blood cell membrane lipid peroxidation in haemodialysis patients, Nephron, 36, pp. 235-237, (1984)
[10]  
Trznadel, Pawlicki, Kedziora, Luciak, Blaszczyk, Buczynski, Superoxide anion generation, erythrocyte superoxide dismutase activity, and lipid peroxidation during hemoperfusion and hemodialysis in chronic uremic patients, Free Radic. Biol. Med., 6, pp. 393-397, (1989)
1234