KINETIC AND ULTRAVIOLET SPECTROSCOPIC STUDIES OF ACTIVE-SITE MUTANTS OF DELTA-5-3-KETOSTEROID ISOMERASE

被引：155
作者：
KULIOPULOS, A
MILDVAN, AS
SHORTLE, D
TALALAY, P
机构：
[1] JOHNS HOPKINS UNIV,SCH MED,DEPT BIOL CHEM,BALTIMORE,MD 21205
[2] JOHNS HOPKINS UNIV,SCH MED,DEPT PHARMACOL & MOLEC SCI,BALTIMORE,MD 21205
来源：
BIOCHEMISTRY | 1989年 / 28卷 / 01期
关键词：
D O I：
10.1021/bi00427a022
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
引用
收藏
页码：149 / 159
页数：11
相关论文
共 52 条
[1]  
Ackers G K, 1970, Adv Protein Chem, V24, P343, DOI 10.1016/S0065-3233(08)60245-4
[2]  
Alder R. W., 1971, MECHANISMS ORGANIC C, P248
[3]  
Batzold F H, 1976, Adv Enzyme Regul, V14, P243, DOI 10.1016/0065-2571(76)90016-9
[4]   PROTON NUCLEAR MAGNETIC-RESONANCE STUDIES OF PSEUDOMONAS-TESTOSTERONI 3-OXO-DELTA-5-STEROID ISOMERASE AND ITS INTERACTION WITH 17-BETA-ESTRADIOL [J].
BENISEK, WF ;
OGEZ, JR .
BIOCHEMISTRY, 1982, 21 (23) :5816-5825
[5]  
BENSON AM, 1975, J BIOL CHEM, V250, P276
[6]   IRREVERSIBLE ACTIVE-SITE-DIRECTED INHIBITION OF DELTA-5-3-KETOSTEROID ISOMERASE BY STEROIDAL 17-BETA-OXIRANES - EVIDENCE FOR 2 MODES OF BINDING IN STEROID-ENZYME COMPLEXES [J].
BEVINS, CL ;
KAYSER, RH ;
POLLACK, RM ;
EKIKO, DB ;
SADOFF, S .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1980, 95 (03) :1131-1137
[7]   MODIFICATION OF AN ENZYME CARBOXYLATE RESIDUE IN THE INHIBITION OF 3-OXO-DELTA-5-STEROID ISOMERASE BY (3S)-SPIRO[5-ALPHA-ANDROSTANE-3,2'-OXIRANE]-17-BETA-OL - IMPLICATIONS FOR THE MECHANISM OF ACTION [J].
BEVINS, CL ;
BANTIA, S ;
POLLACK, RM ;
BOUNDS, PL ;
KAYSER, RH .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1984, 106 (17) :4957-4962
[8]   DETECTION OF A TRANSIENT ENZYME STEROID COMPLEX DURING ACTIVE-SITE-DIRECTED IRREVERSIBLE INHIBITION OF 3-OXO-DELTA-5-STEROID ISOMERASE [J].
BEVINS, CL ;
POLLACK, RM ;
KAYSER, RH ;
BOUNDS, PL .
BIOCHEMISTRY, 1986, 25 (18) :5159-5164
[9]   USE OF ISOTOPE EFFECTS TO DEDUCE THE CHEMICAL MECHANISM OF FUMARASE [J].
BLANCHARD, JS ;
CLELAND, WW .
BIOCHEMISTRY, 1980, 19 (19) :4506-4513
[10]   AFFINITY ALKYLATION OF 3-OXO- DELTA-5-STEROID ISOMERASE BY STEROIDAL 3-BETA-OXIRANES - IDENTIFICATION OF THE MODIFIED AMINO-ACID BY REDUCTION WITH HYDROXYBOROHYDRIDE [J].
BOUNDS, PL ;
POLLACK, RM .
BIOCHEMISTRY, 1987, 26 (08) :2263-2269
123456