SEPARATION OF 2 BETA-GAMMA-SUBUNIT COMPLEXES OF BRAIN GTP-BINDING PROTEINS COMPOSED OF DISTINCT GAMMA-SUBUNITS

被引：12

作者：

ASANO, T ^{[1
]}

MORISHITA, R ^{[1
]}

KOBAYASHI, T ^{[1
]}

KATO, K ^{[1
]}

机构：

[1] MED & BIOL LABS,INA,NAGANO 396,JAPAN

来源：

FEBS LETTERS | 1990年 / 266卷 / 1-2期

关键词：

GTP-binding protein; Phosphorylation; Protein kinase C; γ Subunit;

D O I：

10.1016/0014-5793(90)81501-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The γ subunits of GTP-binding proteins are always associated with β subunits under physiological conditions, and at least two γ subunits exist in the brain. We report here that brain βγ subunit complexes composed of distinct γ subunits can be separated by anion exchange chromatography under nondenaturing conditions. One βγ complex was composed of a 36-kDa β subunit and a 6-kDa γ subunit and the other was composed of 36/35-kDa β subunits and 4.5-kDa γ subunit. The 6-kDa γ subunit was phosphorylated by protein kinase C but the 4.5-kDa γ subunit was not. © 1990.

引用

页码：41 / 44

页数：4

共 24 条

[1] IMMUNOASSAY FOR THE BETA-GAMMA-SUBUNITS OF GTP-BINDING PROTEINS AND THEIR REGIONAL DISTRIBUTION IN BOVINE BRAIN [J].