COMPUTER-ASSISTED METHOD FOR DETERMINATION OF RESTRICTION ENZYME RECOGNITION SITES

被引：58

作者：

GINGERAS, TR ^{[1
]}

MILAZZO, JP ^{[1
]}

ROBERTS, RJ ^{[1
]}

机构：

[1] SUNY STONY BROOK,CTR COMPUTING,STONY BROOK,NY 11794

来源：

NUCLEIC ACIDS RESEARCH | 1978年 / 5卷 / 11期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

D O I：

10.1093/nar/5.11.4105

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A computer program has been developed which aids in the determination of restriction enzyme recognition sequences. This is achieved by cleaving DNAs of known sequence with a restriction endonuclease and comparing the fragmentation pattern with a computer-generated set of patterns. The feasibility of this approach has been tested using fragmentation patterns of øX174 DNA produced by enzymes of both known and unknown specificity. Recognition sequences are predicted for two restriction endonucleases (BbvI and SfaNI) using this method. In addition, recognition sequences are predicted for two other new enzymes (PvuI and MstI) using another computer-assisted method. © 1978 Information Retrieval Limited 1 Falconberg Court London W1V 5FG England.

引用

页码：4105 / 4127

页数：23

共 23 条

[1] MAPPING AND SEQUENCE DETERMINATION OF SINGLE SITE IN PHIX174AM3 REPLICATIVE FORM DNA CLEAVED BY RESTRICTION ENDONUCLEASE PST-I [J].