ALTERATION OF PHOSPHOLIPASE C-DELTA PROTEIN LEVEL AND SPECIFIC ACTIVITY IN ALZHEIMERS-DISEASE

被引：0

作者：

SHIMOHAMA, S

FUJIMOTO, S

MATSUSHIMA, H

TAKENAWA, T

TANIGUCHI, T

PERRY, G

WHITEHOUSE, PJ

KIMURA, J

机构：

[1] KYOTO PHARMACEUT UNIV,DEPT BIOCHEM,KYOTO,JAPAN

[2] KYOTO PHARMACEUT UNIV,DEPT NEUROBIOL,KYOTO,JAPAN

[3] UNIV TOKYO,INST MED,DEPT ONCOBIOL,MINATO KU,TOKYO,JAPAN

[4] CASE WESTERN RESERVE UNIV HOSP,INST PATHOL,DIV NEUROPATHOL,CLEVELAND,OH 44106

[5] CASE WESTERN RESERVE UNIV HOSP,DEPT NEUROL,CLEVELAND,OH 44106

来源：

JOURNAL OF NEUROCHEMISTRY | 1995年 / 64卷 / 06期

关键词：

ALZHEIMERS DISEASE; NEUROFIBRILLARY TANGLES; PHOSPHOLIPASE C-DELTA; ANTIBODY; CONCENTRATION; ACTIVITY; INACTIVATION;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphoinositide-specific phospholipase C (PLC) is a key enzyme in signal transduction. We have previously demonstrated that an antibody to an isozyme of PLC, PLC-delta, produced intense staining of neurofibrillary tangles in the brains of patients with Alzheimer's disease. In the present study, we investigated the protein level and activity of this enzyme in control and Alzheimer brains. Western blot analysis using a specific antibody for PLC-delta showed that the concentration of PLC-delta protein was significantly higher in the cytosolic fraction of Alzheimer's disease cortical tissue than in control brains. The activity of PLC-delta, which hydrolyzes phosphatidylinositol, was also investigated, and we found that PLC-delta activity was not significantly different in the Alzheimer and control cytosolic fractions. These results indicate that the specific activity of PLC-delta is decreased in Alzheimer brains and suggest that inactivation of PLC-delta might be related to the pathophysiology of this disease.

引用

页码：2629 / 2634

页数：6

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