NIST validation studies on the 3500 Genetic Analyzer

被引：10

作者：

Butts, Erica L. R. ^{[1
]}

Kline, Margaret C. ^{[1
]}

Duewer, David L. ^{[1
]}

Hill, Carolyn R. ^{[1
]}

Butler, John M. ^{[1
]}

Vallone, Peter M. ^{[1
]}

机构：

[1] US Natl Inst Stand & Technol, Gaithersburg, MD USA

来源：

FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES | 2011年 / 3卷 / 01期

关键词：

3500 Genetic Analyzer; Capillary electrophoresis; CE; Validation; Threshold; LOQ;

D O I：

10.1016/j.fsigss.2011.08.092

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

An internal validation of the Applied Biosystems 8-capillary 3500 Genetic Analyzer was performed using two commercial short tandem repeat (STR) multiplex kits (Identifiler and Identifiler Plus). Validation experiments to evaluate the performance of this CE consisted of a precision study, sensitivity study, genotype concordance, a two person mixture study, and signal normalization evaluation. The sensitivity study consisted of duplicate injections of amplified DNA template ranging from 1.0 ng down to 0.01 ng. These male samples were heterozygous at all loci. Statistical evaluation of the sensitivity data was performed to determine the statistical significance between employing a single threshold across all dye channels and opting for dye specific thresholds. Published by Elsevier Ireland Ltd.

引用

页码：E184 / E185

页数：2

共 7 条

[1] Applied Biosystems, 2006, AMPFLSTR ID PCR AMPL
[2] Applied Biosystems, 2009, AMPFISTR ID PLUS PCR
[3] Applied Biosystems, 2011, US B APPL BIOS 3500
[4] ARMBRUSTER DA, 1994, CLIN CHEM, V40, P1233
[5] Ellison SLR, 2009, PRACTICAL STATISTICS FOR THE ANALYTICAL SCIENTIST: A BENCH GUIDE, SECOND EDITION, P1
[6] Run-specific limits of detection and quantitation for STR-based DNA testing
Gilder, Jason R.
Doom, Travis E.
Inman, Keith
Krane, Dan E.
[J]. JOURNAL OF FORENSIC SCIENCES, 2007, 52 (01) : 97 - 101
[7] SWGDAM, 2004, FORENSIC SCI COMMUNI, V6

← 1 →