M.H2I, A MULTISPECIFIC 5C-DNA METHYLTRANSFERASE ENCODED BY BACILLUS-AMYLOLIQUEFACIENS PHAGE-H2

被引：20

作者：

LANGE, C

NOYERWEIDNER, M

TRAUTNER, TA

WEINER, M

ZAHLER, SA

机构：

[1] MAX PLANCK INST MOLEC GENET,W-1000 BERLIN 33,GERMANY

[2] CORNELL UNIV,GENET & DEV SECT,ITHACA,NY 14853

来源：

GENE | 1991年 / 100卷

关键词：

RECOMBINANT DNA; NUCLEOTIDE SEQUENCING; GENE EVOLUTION; M.RHO-11SI; AMINO ACID SEQUENCE HOMOLOGY; TARGET-RECOGNIZING DOMAIN;

D O I：

10.1016/0378-1119(91)90369-M

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Bacillus amyloliquefaciens phage H2 codes for a multispecific cytosine-5-DNA-methyltransferase (MTase), M.H2I, which methylates GGCC, GCNGC and [GRAPHICS] target sequences. The gene coding for M.H2I was cloned in Escherichia coli and its nucleotide (nt) sequence was determined. It consists of 1509 bp, corresponding to a protein of 503 amino acids (aa) with a calculated M(r) of 57166. A comparison of the aa sequence of M.H2I with those of the multispecific MTases encoded by Bacillus subtilis phages SPR, phi-3T and rho-11s, revealed that M.H2I is closely related to these enzymes. A very high degree of homology was observed between M.H2I and M.rho-11(s)I, with 96.2% aa identity and 97.8% nt identity of the corresponding genes.

引用

页码：213 / 218

页数：6

共 20 条

[1] CONSTRUCTION AND USE OF CHIMERIC SPR PHI-3T DNA METHYLTRANSFERASES IN THE DEFINITION OF SEQUENCE RECOGNIZING ENZYME REGIONS [J].