PROTEIN DIGESTION IN ISOLATED LYSOSOMES INHIBITED BY INTRALYSOSOMAL TRYPAN BLUE

被引：22

作者：

DAVIES, M

LLOYD, JB

BECK, F

机构：

[1] Welsh National School of Medicine, Tenovus Institute for Cancer Research, The Heath, Cardiff

[2] Department of Biochemistry, University College, Cardiff

[3] Department of Anatomy and Histology, London Hospital Medical College, London E.1, Turner St.

来源：

SCIENCE | 1969年 / 163卷 / 3874期

关键词：

D O I：

10.1126/science.163.3874.1454

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Control rats and rats treated with subcutaneous trypan blue were injected intravenously with denatured albumin-I125. Lysosome-rich fractions of their livers, when incubated at 22°C in osmotically protected medium (pH 7.4), retained their capacity to digest albumin-I125. The rate of digestion was lower in suspensions prepared from rats treated with trypan blue than in control suspensions, but rates of lysosome breakage were not different. These results and other experimental evidence suggest that trypan blue concentrated within lysosomes can inhibit intralysosomal digestion, probably by inhibition of lysosomal proteinases.

引用

页码：1454 / &

共 9 条

[1]

BARKA T, 1961, LAB INVEST, V10, P590

[2] LYSOSOMAL ENZYME INHIBITION BY TRYPAN BLUE - A THEORY OF TERATOGENESIS [J].