DIVERSITY AND COMPLEXITY OF UTERINE MESSENGER-RNA FROM RATS OF DIFFERING HORMONAL STATES

被引：11
作者：
AZIZ, S [1 ]
BALMAIN, A [1 ]
KNOWLER, JT [1 ]
机构：
[1] BEATSON INST CANC RES,GLASGOW G61 1BD,SCOTLAND
来源：
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1979年 / 100卷 / 01期
关键词：
D O I：
10.1111/j.1432-1033.1979.tb02036.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The diversity and complexity of the uterine mRNA population has been compared at two different stages of uterine growth and development in the rat. Analysis by cDNA hybridization to homologous mRNA indicates that there are 8000 different sequences expressed in the immature rat uterus responding to just 4 h of oestradiol‐17β‐induced growth while the fully developed uterus expresses 36000 sequences. As a check on our results, the complexity is re‐analysed by hybridization of mercurated mRNA to total unique DNA. Analysis by this method indicates that the hormone‐stimulated immature rat uterus contains 12000 poly(A)‐containing mRNA sequences while the fully differentiated adult tissue contains 53000 diverse sequences. Copyright © 1979, Wiley Blackwell. All rights reserved
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页码：85 / 94
页数：10
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