NONISOTOPIC HYBRIDIZATION ASSAY FOR DETERMINATION OF RELATIVE AMOUNTS OF GENOTYPIC HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ZIDOVUDINE RESISTANCE

被引：19

作者：

EASTMAN, PS ^{[1
]}

BOYER, E ^{[1
]}

MOLE, L ^{[1
]}

KOLBERG, J ^{[1
]}

URDEA, M ^{[1
]}

HOLODNIY, M ^{[1
]}

机构：

[1] VET AFFAIRS MED CTR,CTR AIDS RES,PALO ALTO,CA 94304

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 10期

关键词：

D O I：

10.1128/JCM.33.10.2777-2780.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A nonisotopic hybridization assay for human immunodeficiency virus genotypic zidovudine resistance determination is described. Biotinylated PCR product was hybridized with enzyme labeled probes for,wild-type or resistant mutant sequences and detected colorimetrically or chemiluminescently in a microplate format. Changes in mutant-to-wild-type ratios allow for the monitoring of longitudinal patient samples.

引用

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页码：2777 / 2780

页数：4

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