POLYOMA-VIRUS TRANSCRIPTION EARLY DURING PRODUCTIVE INFECTION OF MOUSE 3T6-CELLS

Nuclear and cytoplasmic RNAs were isolated from mouse 3T6 cells at different times early in lytic infection by polyoma virus. These RNAs were then annealed to the E and the L strands of 32P-labelled viral DNA prepared in vitro by nick-translation. RNAs complementary to both DNA strands were found in the nuclei and cytoplasm of the cells before viral DNA synthesis was detectable by DNA-DNA filter hybridization. Some of the initial E and L strand viral RNAs detected in the nuclei of the cells were longer than a complete transcript of the viral genome. © 1979.