RECOVERY OF TRANSGENIC CHRYSANTHEMUM (DENDRANTHEMA-GRANDIFLORA TZVELEV) AFTER HYGROMYCIN RESISTANCE SELECTION

被引：39

作者：

RENOU, JP ^{[1
]}

BROCHARD, P ^{[1
]}

JALOUZOT, R ^{[1
]}

机构：

[1] STE CHALLET HERAULT CREAT, F-49340 TREMENTINES, FRANCE

来源：

PLANT SCIENCE | 1993年 / 89卷 / 02期

关键词：

CHRYSANTHEMUM; TRANSFORMATION; AGROBACTERIUM; HYGROMYCIN RESISTANCE;

D O I：

10.1016/0168-9452(93)90127-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have recovered transgenic chrysanthemum plants on a medium containing hygromycin after Agrobacterium-mediated transformation. We used the disarmed hypervirulent strain EHA101 containing a binary vector carrying the neomycine phosphotransferase II, the glucuronidase (GUS) and the hygromycin phosphotransferase genes. Different protocols of transformation are discussed. Optimal conditions were inoculations of nodes (dropping 20 min in the bacterial suspension) which were immediately transferred on a regeneration medium containing cefotaxime (500 mug/ml) and hygromycin (10 mug/ml). Neoformation of buds occurred on selective medium within two months on almost 40% of the inoculated explants. DNA analysis of plants showing GUS expression confirmed the T-DNA integration in the plant genome.

引用

页码：185 / 197

页数：13

共 22 条

[1]

ANDERSON NO, 1988, EUPHYTICA, V37, P229

[2]

ANDERSON NO, 1987, HORTSCIENCE, V313, P22

[3]

BARFIELD DG, 1991, PLANT CELL REP, V10, P308, DOI 10.1007/BF00193148

[4]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[5] MOLECULAR-CLONING OF OVERLAPPING SEGMENTS OF THE NOPALINE TI-PLASMID PTIC58 AS A MEANS TO RESTRICTION ENDONUCLEASE MAPPING [J].

DEPICKER, A ;

DEWILDE, M ;

DEVOS, G ;

DEVOS, R ;

VANMONTAGU, M ;

SCHELL, J .

PLASMID, 1980, 3 (02) :193-211

[6] BROAD HOST RANGE DNA CLONING SYSTEM FOR GRAM-NEGATIVE BACTERIA - CONSTRUCTION OF A GENE BANK OF RHIZOBIUM-MELILOTI [J].

DITTA, G ;

STANFIELD, S ;

CORBIN, D ;

HELINSKI, DR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (12) :7347-7351

[7] USE OF THE GUS GENE AS A SELECTABLE MARKER FOR AGROBACTERIUM-MEDIATED TRANSFORMATION OF RUBUS [J].

GRAHAM, J ;

MCNICOL, RJ ;

KUMAR, A .

PLANT CELL TISSUE AND ORGAN CULTURE, 1990, 20 (01) :35-39

[8] THE HYPERVIRULENCE OF AGROBACTERIUM-TUMEFACIENS A281 IS ENCODED IN A REGION OF PTIBO542 OUTSIDE OF TRANSFER DNA [J].

HOOD, EE ;

HELMER, GL ;

FRALEY, RT ;

CHILTON, MD .

JOURNAL OF BACTERIOLOGY, 1986, 168 (03) :1291-1301

[9] A SIMPLE AND GENERAL-METHOD FOR TRANSFERRING GENES INTO PLANTS [J].

HORSCH, RB ;

FRY, JE ;

HOFFMANN, NL ;

EICHHOLTZ, D ;

ROGERS, SG ;

FRALEY, RT .

SCIENCE, 1985, 227 (4691) :1229-1231

[10]

JAMES DJ, 1989, PLANT CELL REP, V7, P658, DOI 10.1007/BF00272054

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