CALCIUM-INDUCED POTASSIUM PATHWAY IN SIDED ERYTHROCYTE-MEMBRANE VESICLES

被引:16
作者
SZE, H [1 ]
SOLOMON, AK [1 ]
机构
[1] HARVARD UNIV,SCH MED,BIOPHYS LAB,BOSTON,MA 02115
关键词
(Erythrocyte membrane vesicle); Ca[!sup]2+[!/sup; K[!sup]+[!/sup; Permeability; Sidedness;
D O I
10.1016/0005-2736(79)90017-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have characterized the asymmetric effect of Ca2+ on passive K+ permeability in erythrocyte membranes, using inside out and right-side out vesicles. Ca2+, but not Mg2+, can induce an increase in K+ uptake in inside out vesicles. The half-maximal concentration of Ca2+ required to induce the K+ uptake is 0.2 mM, and the permeability increase is not specific for K+. Thus, the Ca2+-induced permeation process in inside out vesicles is changed from that in the energy-depleted intact cell which requires only micromolar concentrations of Ca2+ and is specific for K+. Removal of spectrin had no effect on the vesicle permeability increase due to Ca2+. Studies with N-ethylmaleimide show that the vesicle channel opening is mediated by a protein and passage is controlled by sulfhydryl groups; furthermore, the Ca2+-induced vesicle pathway is distinct from the normal channel for passive K+ leak in the absence of Ca2+. The protein is sensitive to its phospholipid environment since removal of easily accessible phospholipid head groups on the cytoplasmic face of the vesicles inhibits the Ca2+-stimulated channel opening. © 1979.
引用
收藏
页码:180 / 194
页数:15
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