INHIBITION OF PHOSPHOLIPASE-A2 PURIFIED FROM HUMAN HERNIATED DISK

被引：12

作者：

CARABAZA, A ^{[1
]}

CABRE, F ^{[1
]}

GARCIA, AM ^{[1
]}

GOMEZ, M ^{[1
]}

SANCHEZ, J ^{[1
]}

MAULEON, D ^{[1
]}

CARGANICO, G ^{[1
]}

机构：

[1] LABS MENARINI SA, DEPT R&D, ALFONSO XII 587, E-08912 BADALONA, SPAIN

来源：

BIOCHEMICAL PHARMACOLOGY | 1993年 / 45卷 / 03期

关键词：

D O I：

10.1016/0006-2952(93)90158-S

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The effect on human herniated intervertebral disc phospholipase A2 (HD-PLA2) of a number of retinoids, antirheumatic drugs and reported PLA2 inhibitors was evaluated using autoclaved [1-C-14]oleate-labeled Escherichia coli membranes as the substrate. Dexamethasone, non-steroidal antiinflammatory drugs, aristolochic acid and retinol were inactive, whereas a marked inhibition was found for manoalide, retinal, nordihydroguaiaretic acid and p-bromophenacyl bromide after preincubation with the enzyme (IC50 values 0.25, 4, 5 and 5 muM, respectively). The results are parallel to those obtained with the PLA2 purified from human synovial fluid.

引用

页码：783 / 786

页数：4

共 27 条

[1] DIFFERENTIAL-EFFECTS OF MANOALIDE ON SECRETED AND INTRACELLULAR PHOSPHOLIPASES
BENNETT, CF
MONG, S
CLARKE, MA
KRUSE, LI
CROOKE, ST
[J]. BIOCHEMICAL PHARMACOLOGY, 1987, 36 (05) : 733 - 740
[2] BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911
[3] INFLAMMATION AND COLLAGENASE PRODUCTION IN RATS WITH ADJUVANT ARTHRITIS REDUCED WITH 13-CIS-RETINOIC ACID
BRINCKERHOFF, CE
COFFEY, JW
SULLIVAN, AC
[J]. SCIENCE, 1983, 221 (4612) : 756 - 758
[4] BURLEY ES, 1982, PHARMACOLOGIST, V24, P117
[5] DIFFERENTIAL HYDROLYSIS OF 1-ALKYL-2-ACYL AND DIACYLGLYCEROPHOSPHOCHOLINES BY HUMAN AND NONHUMAN PHOSPHOLIPASES-A(2)
CABRE, F
GARCIA, AM
CARABAZA, A
MAULEON, D
CARGANICO, G
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1124 (03) : 297 - 299
[6] Dennis EA., 1983, ENZYMES, P307
[7] INHIBITION OF HUMAN NON-PANCREATIC PHOSPHOLIPASES-A2 BY RETINOIDS AND FLAVONOIDS - MECHANISM OF ACTION
FAWZY, AA
VISHWANATH, BS
FRANSON, RC
[J]. AGENTS AND ACTIONS, 1988, 25 (3-4): : 394 - 400
[8] FIEDLER-NAGY C, 1987, Dermatologica (Basel), V175, P81
[9] FRANSON R, 1974, J LIPID RES, V15, P380
[10] DOSE-DEPENDENT SUPPRESSION BY THE SYNTHETIC RETINOID, 4-HYDROXYPHENYL RETINAMIDE, OF STREPTOCOCCAL CELL WALL-INDUCED ARTHRITIS IN RATS
HARAOUI, B
WILDER, RL
ALLEN, JB
SPORN, MB
HELFGOTT, RK
BRINCKERHOFF, CE
[J]. INTERNATIONAL JOURNAL OF IMMUNOPHARMACOLOGY, 1985, 7 (06): : 903 - &

← 1 2 3 →