DECREASE IN HEART MITOCHONDRIAL CREATINE-KINASE ACTIVITY DUE TO OXYGEN FREE-RADICALS

被引：56

作者：

YUAN, GX ^{[1
]}

KANEKO, M ^{[1
]}

MASUDA, H ^{[1
]}

HON, RB ^{[1
]}

KOBAYASHI, A ^{[1
]}

YAMAZAKI, N ^{[1
]}

机构：

[1] HAMAMATSU UNIV SCH MED, DEPT INTERNAL MED 3, HAMAMATSU, SHIZUOKA 431-31, JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1140卷 / 01期

关键词：

CREATINE KINASE; SULFHYDRYL GROUP; FREE RADICAL; SUPEROXIDE ANION RADICAL; HYDROGEN PEROXIDE; (RAT HEART MITOCHONDRION);

D O I：

10.1016/0005-2728(92)90022-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study was undertaken to examine the effects of oxygen free radicals on mitochondrial creatine kinase activity in rat heart. Xanthine plus xanthine oxidase (superoxide anion radical generating system) reduced mitochondrial creatine kinase activity both in a dose- and a time-dependent manner. Superoxide dismutase showed a protective effect on depression in creatine kinase activity due to xanthine plus xanthine oxidase. Hydrogen peroxide inhibited creatine kinase activity in a dose-dependent manner, this inhibition was protected by the addition of catalase. In order to understand the detailed mechanisms by which oxygen free radicals inhibit mitochondrial creatine kinase activity, the effects of oxygen free radicals on mitochondrial sulfhydryl groups were examined. Mitochondrial sulfhydryl groups contents were decreased by xanthine plus xanthine oxidase or hydrogen peroxide; this depression in sulfhydryl groups contents was prevented by the addition of superoxide dismutase or catalase. N-Ethylmaleimide (sulfhydryl group reagent) expressed inhibitory effects on the creatine kinase activity both in a dose- and a time-dependent manner; dithiothreitol or cysteine (sulfhydryl group reductant) showed protective effects on the creatine kinase activity depression induced by N-ethylmaleimide. Dithiothreitol or cysteine also blocked the depression of mitochondrial creatine kinase activity caused by xanthine plus xanthine oxidase or hydrogen peroxide. These results lead us to conclude that oxygen free radicals may inhibit mitochondrial creatine kinase activity by modifying sulfhydryl groups in the enzyme protein.

引用

页码：78 / 84

页数：7

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