The time-course and RNA interference of TNF-alpha, IL-6, and IL-1 beta expression on neuropathic pain induced by L5 spinal nerve transection in rats

被引：22

作者：

Choi, Byung Moon ^{[1
]}

Lee, Soo Han ^{[2
]}

An, Sang Mee ^{[1
]}

Park, Do Yang ^{[2
]}

Lee, Gwan Woo ^{[3
]}

Noh, Gyu-Jeong ^{[1
,2
]}

机构：

[1] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Anesthesiol & Pain Med, 88,Olympic Ro 43-Gil, Seoul 138736, South Korea

[2] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Clin Pharmacol & Therapeut, Seoul 138736, South Korea

[3] Dankook Univ, Coll Med, Dept Anesthesiol & Pain Med, Cheonan, South Korea

来源：

KOREAN JOURNAL OF ANESTHESIOLOGY | 2015年 / 68卷 / 02期

关键词：

Cytokine; Neuropathic pain; RNA interference;

D O I：

10.4097/kjae.2015.68.2.159

中图分类号：

R614 [麻醉学];

学科分类号：

100217 ;

摘要：

Background: The objective of this study was to investigate the time-course of the expression of TNF-alpha, IL-6, and IL-1 beta after L5 spinal nerve transection (SNT), and to determine the effect of small interfering RNA (siRNA) targeting these cytokines on neuropathic pain. Methods: Rats received control siRNA (CON group, n = 80) or a cocktail of siRNAs targeting these cytokines (COCK group, n = 70). The siRNAs were given via intrathecal catheter 1 d prior to SNT, on the operation day, and 1, 2 and 3 d postoperatively. Behavioral tests and levels of the cytokine mRNAs and proteins as well as glial cell activity were following the L5 SNT. Results: In the CON group, TNF-alpha and IL-1 beta mRNA levels increased immediately after SNT and remained high for 6 d, while IL-6 transcripts only began to increase after 12 h. TNF-alpha and IL-1 beta mRNA levels in the COCK group were lower than in the CON group at all time points (P < 0.05). In the behavioral tests, allodynia and hyperalgesia were significantly lower in the COCK group from 2 d after SNT (P < 0.05). Conclusions: The time courses of TNF-alpha, IL-6 and IL-1 beta mRNA expression after L5 SNT differ. RNA interference may be a method of reducing the development of mechanical allodynia and hyperalgesia in response to nerve injury.

引用

页码：159 / 169

页数：11

共 28 条

[1] Intrathecal anti-IL-6 antibody and IgG attenuates peripheral nerve injury-induced mechanical allodynia in the rat: possible immune modulation in neuropathic pain
Arruda, JL
Sweitzer, SA
Rutkowski, MD
DeLeo, JA
[J]. BRAIN RESEARCH, 2000, 879 (1-2) : 216 - 225
[2] 3′ UTR seed matches, but not overall identity, are associated with RNAi off-targets
Birmingham, A
Anderson, EM
Reynolds, A
Ilsley-Tyree, D
Leake, D
Fedorov, Y
Baskerville, S
Maksimova, E
Robinson, K
Karpilow, J
Marshall, WS
Khvorova, A
[J]. NATURE METHODS, 2006, 3 (03) : 199 - 204
[3] Short interfering RNA (siRNA): tool or therapeutic?
Cejka, D
Losert, D
Wacheck, V
[J]. CLINICAL SCIENCE, 2006, 110 (01) : 47 - 58
[4] Discordant protein and mRNA expression in lung adenocarcinomas
Chen, GA
Gharib, TG
Huang, CC
Taylor, JMG
Misek, DE
Kardia, SLR
Giordano, TJ
Iannettoni, MD
Orringer, MB
Hanash, SM
Beer, DG
[J]. MOLECULAR & CELLULAR PROTEOMICS, 2002, 1 (04) : 304 - 313
[5] Chung JM, 2004, METH MOLEC MED, P35
[6] The effect of site and type of nerve injury on spinal glial activation and neuropathic pain behavior
Colburn, RW
Rickman, AJ
DeLeo, JA
[J]. EXPERIMENTAL NEUROLOGY, 1999, 157 (02) : 289 - 304
[7] Interleukin-6-mediated hyperalgesia/allodynia and increased spinal IL-6 expression in a rat mononeuropathy model
DeLeo, JA
Colburn, RW
Nichols, M
Malhotra, A
[J]. JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 1996, 16 (09) : 695 - 700
[8] Dorazil-Dudzik M, 2004, ANESTH ANALG, V98, P1566, DOI 10.1213/01.ANE.0000113235.88534.48
[9] siRNA relieves chronic neuropathic pain
Dorn, G
Patel, S
Wotherspoon, G
Hemmings-Mieszczak, M
Barclay, J
Natt, FJC
Martin, P
Bevan, S
Fox, A
Ganju, P
Wishart, W
Hall, J
[J]. NUCLEIC ACIDS RESEARCH, 2004, 32 (05) : e49
[10] CUTANEOUS HYPERALGESIA INDUCED BY PERIPHERAL INJECTION OF INTERLEUKIN-1-BETA IN THE RAT
FUKUOKA, H
KAWATANI, M
HISAMITSU, T
TAKESHIGE, C
[J]. BRAIN RESEARCH, 1994, 657 (1-2) : 133 - 140

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