DETERMINATION OF ORIENTATION AND POSITION OF MYOGLOBIN MOLECULE IN CRYSTAL OF SEAL MYOGLOBIN

被引：18

作者：

TOLLIN, P

机构：

[1] Department of Biological Sciences, Purdue University, Lafayette, IN

[2] Carnegie Laboratory of Physics, The University, Dundee, Scotland

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1969年 / 45卷 / 03期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

D O I：

10.1016/0022-2836(69)90307-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The orientation of the myoglobin molecule in the crystal of seal myoglobin has been determined, by using the rotation function (Rossmann & Blow, 1962) to compare the 5.8 Å resolution data from sperm whale myoglobin with the 5 Å data from seal myoglobin obtained by Scouloudi (1969). The position of the molecule in the unit cell has been found using the Q-functions defined by Tollin (1966a). The position of the haem group obtained in this way is in good agreement with that obtained by Scouloudi (1969) from a 5 Å resolution electron density map obtained using the isomorphous replacement map. A three-dimensional electron density map has been obtained by calculating phases for a whale myoglobin molecule correctly located in the seal myoglobin cell and applying these to the observed seal structure amplitudes. This map has been compared with Scouloudi's published results and indicates a good agreement between the two investigations. © 1969.

引用

页码：481 / &

共 15 条

[1] THE CRYSTAL STRUCTURE OF MYOGLOBIN .5. A LOW-RESOLUTION 3-DIMENSIONAL FOURIER SYNTHESIS OF SPERM-WHALE MYOGLOBIN CRYSTALS [J].