CA-ATPASE ISOZYME EXPRESSION IN SARCOPLASMIC-RETICULUM IS ALTERED BY CHRONIC STIMULATION OF SKELETAL-MUSCLE

被引：67
作者：
BRIGGS, FN
LEE, KF
FEHER, JJ
WECHSLER, AS
OHLENDIECK, K
CAMPBELL, K
机构：
[1] VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT SURG,RICHMOND,VA 23298
[2] UNIV IOWA HOSP & CLIN,DEPT PHYSIOL & BIOPHYS,IOWA CITY,IA 52242
来源：
FEBS LETTERS | 1990年 / 259卷 / 02期
关键词：
Ca uptake; Ca-ATPase isozyme: (Ruthenium red); Sarcoplasmic reticulum; Skeletal muscle transformation;
D O I：
10.1016/0014-5793(90)80025-E
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Chronic stimulation of a predominantly fast skeletal muscle enhanced the expression of type I (slow muscle) Ca-ATPase and suppressed the expression of the type II (fast muscle) Ca-ATPase. Monoclonal antibodies IID8 and IIH11 against type I (slow) and type II (fast) isozymes respectively, were used to type the Ca-ATPases of the isolated SR (sarcoplasmic reticulum) by Western blots, and the Ca-ATPases of the muscle fibers by immunohistochemistry. Of the fibers from control muscles 80% stained for the type II isozyme and 20% for the type I isozyme. Following chronic stimulation all fibers stained for type I isozyme and none stained for type II isozyme. Ca-ATPase isozyme distribution in isolated SR confirmed this effect of chronic stimulation. The calcium uptake activities of homogenates of stimulated muscles were 22% of the control muscles. The Ca-ATPase and calcium-uptake activities of the isolated SR from stimulated muscles were, respectively, 32 and 45% of the control muscles. © 1990.
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页码：269 / 272
页数：4
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