ROLE OF COULOMB ENERGY IN PROMOTING COLLISIONALLY ACTIVATED DISSOCIATION OF MULTIPLY-CHARGED PEPTIDES FORMED BY ELECTROSPRAY-IONIZATION

被引：16
作者：
ISHIKAWA, K
NISHIMURA, T
KOGA, Y
NIWA, Y
机构：
[1] NATL INST MAT & CHEM RES,TSUKUBA,IBARAKI 305,JAPAN
[2] UPJOHN PHARMACEUT LTD,TSUKUBA,IBARAKI 305,JAPAN
来源：
RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1994年 / 8卷 / 12期
关键词：
D O I：
10.1002/rcm.1290081205
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Electrospray ionization tandem quadrupole mass spectrometry has been applied to a series of lysine-substituted octaalanines and some naturally occurring peptides containing more than two basic amino acid residues. Unusually high fragmentation efficiency along with site-specific cleavages at the outer side of and remote from basic residues were observed for collisionally activated dissociation spectra of multiply charged peptides (MCP) with basic residues in close proximity, it was suggested that Coulomb energy (CE) rather than Coulomb repulsion (CR) was responsible for promoting fragmentation of the MCP studied. A possible fragmentation mechanism of MCP is proposed, in which the conversion of CE into internal vibrational energy was brought about by intramolecular proton migration in the presence of CR between charged sites.
引用
收藏
页码：933 / 938
页数：6
相关论文
共 22 条
[1]   SECONDARY ION MASS-SPECTROMETRY WITH CESIUM ION PRIMARY BEAM AND LIQUID TARGET MATRIX FOR ANALYSIS OF BIOORGANIC COMPOUNDS [J].
ABERTH, W ;
STRAUB, KM ;
BURLINGAME, AL .
ANALYTICAL CHEMISTRY, 1982, 54 (12) :2029-2034
[2]   FAST ATOM BOMBARDMENT OF SOLIDS (FAB) - A NEW ION-SOURCE FOR MASS-SPECTROMETRY [J].
BARBER, M ;
BORDOLI, RS ;
SEDGWICK, RD ;
TYLER, AN .
JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1981, (07) :325-327
[3]  
BARINAGA CJ, 1989, RAPID COMMUN MASS SP, V3, P160
[4]   CONTRIBUTIONS OF MASS-SPECTROMETRY TO PEPTIDE AND PROTEIN-STRUCTURE [J].
BIEMANN, K .
BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY, 1988, 16 (1-12) :99-111
[5]   MASS-SPECTROMETRIC DETERMINATION OF THE AMINO-ACID-SEQUENCE OF PEPTIDES AND PROTEINS [J].
BIEMANN, K ;
MARTIN, SA .
MASS SPECTROMETRY REVIEWS, 1987, 6 (01) :1-75
[6]   THE SIMULTANEOUS MULTIPLE PRODUCTION OF SOLUTION PHASE PEPTIDES - ASSESSMENT OF THE GEYSEN METHOD OF SIMULTANEOUS PEPTIDE-SYNTHESIS [J].
BRAY, AM ;
MAEJI, NJ ;
GEYSEN, HM .
TETRAHEDRON LETTERS, 1990, 31 (40) :5811-5814
[7]   IDENTIFICATION OF RECOMBINANT HUMAN LYSOZYME AT THE PICOMOLE LEVEL BY ELECTROSPRAY-LC/MS [J].
Ishikawa, Keiichiro ;
Niwa, Yoshio .
ANALYTICAL SCIENCES, 1991, 7 :967-970
[8]   TANDEM MASS-SPECTROMETRY OF VERY LARGE MOLECULES - SERUM-ALBUMIN SEQUENCE INFORMATION FROM MULTIPLY CHARGED IONS FORMED BY ELECTROSPRAY IONIZATION [J].
LOO, JA ;
EDMONDS, CG ;
SMITH, RD .
ANALYTICAL CHEMISTRY, 1991, 63 (21) :2488-2499
[9]   TANDEM MASS-SPECTROMETRY OF VERY LARGE MOLECULES .2. DISSOCIATION OF MULTIPLY CHARGED PROLINE-CONTAINING PROTEINS FROM ELECTROSPRAY IONIZATION [J].
LOO, JA ;
EDMONDS, CG ;
SMITH, RD .
ANALYTICAL CHEMISTRY, 1993, 65 (04) :425-438
[10]   COLLISIONAL ACTIVATION AND DISSOCIATION OF LARGE MULTIPLY CHARGED PROTEINS PRODUCED BY ELECTROSPRAY IONIZATION [J].
LOO, JA ;
EDMONDS, CG ;
UDSETH, HR ;
SMITH, RD .
ANALYTICA CHIMICA ACTA, 1990, 241 (02) :167-173
123